Subramaniam Deepika, Sekaran Saravanan
General Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Prosthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Feb 21;16(2):e54597. doi: 10.7759/cureus.54597. eCollection 2024 Feb.
Bone tissue engineering (BTE) is an emerging interdisciplinary field that aims to develop new strategies and materials for repairing, regenerating, or replacing damaged bone tissues. This field combines engineering, biology, and medicine principles to create functional bone tissues in the laboratory and in vivo. The main goal of BTE is to create biological substitutes that mimic the structure, function, and properties of natural bone tissue, thereby promoting the regeneration of bone defects caused by trauma, disease, or aging. In this study, we developed a biocomposite membrane using magnesium-chitosan, carboxymethyl cellulose, and alginate through a simple cast drying method. The biocompatibility of the membrane was evaluated using human osteoblastic cells, and it was found to be nontoxic to these cells. Both metabolic activity measurements (24 and 48 hours) and the lactate dehydrogenase release assay (72 hours) indicated that the membrane was biocompatible and did not exert significant toxic effects. These results suggest that the developed biocomposite membrane has the potential to be used as a safe and effective biomaterial for various biomedical applications, such as BTE, wound healing, and drug delivery. Further studies are warranted to explore the full potential of this membrane and its performance in different biological environments.
骨组织工程（BTE）是一个新兴的跨学科领域，旨在开发用于修复、再生或替换受损骨组织的新策略和材料。该领域结合工程学、生物学和医学原理，在实验室和体内创造功能性骨组织。BTE的主要目标是制造出模仿天然骨组织结构、功能和特性的生物替代品，从而促进由创伤、疾病或衰老引起的骨缺损的再生。在本研究中，我们通过简单的流延干燥法，使用镁-壳聚糖、羧甲基纤维素和海藻酸盐制备了一种生物复合膜。使用人成骨细胞评估了该膜的生物相容性，发现其对这些细胞无毒。代谢活性测量（24小时和48小时）以及乳酸脱氢酶释放试验（72小时）均表明该膜具有生物相容性，且未产生明显的毒性作用。这些结果表明，所开发的生物复合膜有潜力作为一种安全有效的生物材料用于各种生物医学应用，如骨组织工程、伤口愈合和药物递送。有必要进行进一步研究，以探索该膜的全部潜力及其在不同生物环境中的性能。
