Heavy Engineering 133, Department of Mechanical Engineering, State University of New York at Stony Brook, Stony Brook, New York, 11794-2300.
LE 153, Department of Mechanical Engineering, State University of New York at Stony Brook, Stony Brook, New York, 11794-2300.
J Biomed Mater Res A. 2017 Dec;105(12):3350-3359. doi: 10.1002/jbm.a.36200. Epub 2017 Sep 19.
This work aims to explore the concentration-dependence of SPIO-Au core-shell nanoscale particles (NPs) (17.3 ± 1.2 nm in diameter) on biocompatibility and osteogenic differentiation of preosteoblast MC3T3-E1 cells. The stability of NPs was first investigated by UV-vis absorption spectra and zeta potential measurement. Then concentration effects of NPs (1-80 μg/mL) were evaluated on viability, morphology, proliferation, cellular uptake, and alkaline phosphate (ALP) activity levels. Results have shown strong stability and no acute toxicity (viability > 93%) or morphological difference at all concentration levels of NPs. The proliferation results indicated that the concentration of NPs below 40 μg/mL does not affect the cell proliferation for 7 days of incubation. Transmission electron microscopy images revealed the successful internalization of NPs into MC3T3-E1 cells and the dose-dependent accumulation of NPs inside the cytoplasm. The ALP level of MC3T3-E1 cells was improved by 49% (of control) after treated with NPs at 10 μg/mL for 10 days, indicating their positive effect on early osteogenic differentiation. This study confirmed the excellent biocompatibility of SPIO-Au NPs and their great potential for promoting osteogenic differentiation and promised the future application for these NPs in bone engineering including drug delivery, cell labeling, and activity tracking within scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3350-3359, 2017.
本研究旨在探索超顺磁性氧化铁-金（SPIO-Au）核壳纳米粒子（NPs）（直径为 17.3±1.2nm）的浓度依赖性对前成骨细胞 MC3T3-E1 细胞的生物相容性和成骨分化的影响。首先通过紫外-可见吸收光谱和 Zeta 电位测量来研究 NPs 的稳定性。然后评估了 NPs（1-80μg/mL）浓度对细胞活力、形态、增殖、细胞摄取和碱性磷酸酶（ALP）活性水平的影响。结果表明，NPs 具有很强的稳定性，且在所有浓度水平下均无急性毒性（活力>93%）或形态差异。增殖结果表明，NPs 浓度低于 40μg/mL 时，在孵育 7 天内不会影响细胞增殖。透射电子显微镜图像显示 NPs 成功地被内化到 MC3T3-E1 细胞内，并且 NPs 在细胞质内呈剂量依赖性积累。用 10μg/mL 的 NPs 处理 10 天后，MC3T3-E1 细胞的 ALP 水平提高了 49%（与对照相比），表明其对早期成骨分化具有积极作用。本研究证实了 SPIO-Au NPs 的优异生物相容性及其在促进成骨分化方面的巨大潜力，并为这些 NPs 在骨工程中的未来应用（包括药物输送、细胞标记和支架内活性跟踪）提供了保证。© 2017 Wiley Periodicals, Inc. J 生物材料 Res 部分 A：105A：3350-3359，2017。
