State Key Laboratory of Organic-inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China; Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
Mater Sci Eng C Mater Biol Appl. 2015 Oct;55:166-73. doi: 10.1016/j.msec.2015.05.002. Epub 2015 May 2.
Proliferation and differentiation of bone-related cells are modulated by many factors such as scaffold design, growth factor, dynamic culture system, and physical simulation. Nanofibrous structure and moderate-intensity (1 mT-1 T) static magnetic field (SMF) have been identified as capable of stimulating proliferation and differentiation of osteoblasts. Herein, magnetic nanofibers were prepared by electrospinning mixture solutions of poly(L-lactide) (PLLA) and ferromagnetic Fe3O4 nanoparticles (NPs). The PLLA/Fe3O4 composite nanofibers demonstrated homogeneous dispersion of Fe3O4 NPs, and their magnetism depended on the contents of Fe3O4 NPs. SMF of 100 mT was applied in the culture of MC3T3-E1 osteoblasts on pure PLLA and PLLA/Fe3O4 composite nanofibers for the purpose of studying the effect of SMF on osteogenic differentiation of osteoblastic cells on magnetic nanofibrous scaffolds. On non-magnetic PLLA nanofibers, the application of external SMF could enhance the proliferation and osteogenic differentiation of MC3T3-E1 cells. In comparison with pure PLLA nanofibers, the incorporation of Fe3O4 NPs could also promote the proliferation and osteogenic differentiation of MC3T3-E1 cells in the absence or presence of external SMF. The marriage of magnetic nanofibers and external SMF was found most effective in accelerating every aspect of biological behaviors of MC3T3-E1 osteoblasts. The findings demonstrated that the magnetic feature of substrate and microenvironment were applicable ways in regulating osteogenesis in bone tissue engineering.
骨相关细胞的增殖和分化受许多因素的调节，如支架设计、生长因子、动态培养系统和物理模拟。纳米纤维结构和中等强度（1 mT-1 T）静磁场（SMF）已被确定为能够刺激成骨细胞的增殖和分化。本文通过静电纺丝聚 L-乳酸（PLLA）和铁磁 Fe3O4 纳米粒子（NPs）的混合溶液制备了磁性纳米纤维。PLLA/Fe3O4 复合纳米纤维表现出 Fe3O4 NPs 的均匀分散，其磁性取决于 Fe3O4 NPs 的含量。在纯 PLLA 和 PLLA/Fe3O4 复合纳米纤维上培养 MC3T3-E1 成骨细胞时施加 100 mT 的 SMF，目的是研究 SMF 对磁性纳米纤维支架上成骨细胞成骨分化的影响。在非磁性 PLLA 纳米纤维上，施加外部 SMF 可以增强 MC3T3-E1 细胞的增殖和成骨分化。与纯 PLLA 纳米纤维相比，在不存在或存在外部 SMF 的情况下，加入 Fe3O4 NPs 也可以促进 MC3T3-E1 细胞的增殖和成骨分化。磁性纳米纤维和外部 SMF 的结合被发现最有效地加速 MC3T3-E1 成骨细胞的各个方面的生物学行为。研究结果表明，基底和微环境的磁性特征是调节骨组织工程中成骨的适用方法。
