Zhou Yanfeng, Li Xueyan, Li Wenyuan, Qin Rong
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
Sheng Wu Gong Cheng Xue Bao. 2017 Jul 25;33(7):1158-1167. doi: 10.13345/j.cjb.170013.
To study the effects of different frequency sinusoidal electromagnetic fields on the bone mineral density and bone histomorphometry of SD young rats, and to screen suitable electromagnetic field frequency. In total 32 female SD rats of 8 weeks old were randomly divided into 4 groups: control group, 15 Hz group, 30 Hz group and 45 Hz group. Except for the control group, rats in experimental groups were treated with corresponding frequency of 1.8 mT sinusoidal electromagnetic field 90 minutes every day. Rats were measured for bone mineral density after 8 weeks by dual energy X-ray absorptiometry. Rats' femur and vertebral bones were measured by analyzing the static and dynamic forms on the right tibia bone for morphometrics. Rat serum was measured to estimate the index of bone formation and bone resorption. Bone mineral density of rats from 15 Hz group and 45 Hz group was higher than that of the control group (P<0.05). Serum osteocalcin level of rats from 15 Hz group and 45 Hz group was higher than that of the control group (P<0.05). Double fluorescence spacing and static parameters of bone tissue in experimental group rat tibia were higher than that of the control group (P<0.05). Our findings imply that 15 Hz and 45 Hz sinusoidal electromagnetic fields can effectively increase bone mineral density in young rats for preventing osteoporosis.
研究不同频率正弦电磁场对SD幼鼠骨密度和骨组织形态计量学的影响，筛选合适的电磁场频率。将32只8周龄雌性SD大鼠随机分为4组：对照组、15Hz组、30Hz组和45Hz组。除对照组外，实验组大鼠每天接受频率为1.8mT的相应频率正弦电磁场处理90分钟。8周后采用双能X线吸收法测量大鼠骨密度。通过分析右侧胫骨骨的静态和动态形态来测量大鼠的股骨和椎骨进行形态计量学分析。检测大鼠血清以评估骨形成和骨吸收指标。15Hz组和45Hz组大鼠的骨密度高于对照组（P<0.05）。15Hz组和45Hz组大鼠血清骨钙素水平高于对照组（P<0.05）。实验组大鼠胫骨骨组织的双荧光间距和静态参数高于对照组（P<0.05）。我们的研究结果表明，15Hz和45Hz正弦电磁场可有效提高幼鼠骨密度，预防骨质疏松。
