Gao Yu-Hai, Zhou Yan-Feng, Li Shao-Feng, Li Wen-Yuan, Xi Hui-Rong, Yang Fang-Fang, Chen Ke-Ming
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China.
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China;
Zhongguo Gu Shang. 2017 Dec 25;30(12):1113-1117. doi: 10.3969/j.issn.1003-0034.2017.12.008.
To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats.
Thirty SD rats weighted(110±10) and aged 1 month were randomly divided into control group and electromagnetic field group, 15 in each group. Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week, and observed food-intake. Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body, and detected bone density of femur and vertebral body. Osteocalcin and tartrate-resistant acid phosphatase 5b were detected by ELSA; weighted liver, kidney and uterus to calculate purtenance index, then detected pathologic results by HE.
Compared with control group, there was no significant change in weight every week, food-intake every day; no obvious change of bone density of whole body at 2 and 4 weeks, however bone density of whole body, bone density of excised femur and vertebra were increased at 6 weeks. Expression of OC was increased, and TRACP 5b expression was decreased. No change of HE has been observed in liver, kidney and uterus and organic index.
50 Hz 1.8 mT sinusoidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs, to improve peak bone density of young rats, in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation.
研究50Hz 1.8mT正弦电磁场（SEMFs）对SD大鼠骨密度（BMD）的影响。
选取30只体重（110±10）g、1月龄的SD大鼠，随机分为对照组和电磁场组，每组15只。正常对照组采用50Hz 0mT强度，正弦电磁场组采用50Hz 1.8mT强度，每天照射1.5h，每周称体重1次，观察进食量。腹腔注射麻醉大鼠，采用双能X线吸收法检测全身骨密度，并检测股骨和椎体骨密度。采用酶联免疫吸附测定法（ELSA）检测骨钙素和抗酒石酸酸性磷酸酶5b；称取肝脏、肾脏和子宫重量并计算脏器指数，然后采用苏木精-伊红（HE）染色观察病理结果。
与对照组相比，每周体重、每日进食量无明显变化；2周和4周时全身骨密度无明显变化，但6周时全身骨密度、离体股骨和椎体骨密度升高。骨钙素表达升高，抗酒石酸酸性磷酸酶5b表达降低。肝脏、肾脏和子宫的HE染色及脏器指数无变化。
50Hz 1.8mT正弦电磁场可促进骨形成，降低骨吸收相关因子，提高幼年大鼠的峰值骨密度，为临床研究电磁场防治骨质疏松奠定基础。
