Laboratoire Innovations technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Gif-Sur-Yvette, France.
Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d'Infection et d'Immunité de Lille, 1 rue du Professeur Calmette, Lille, France.
Proteomics. 2017 Nov;17(21). doi: 10.1002/pmic.201700211. Epub 2017 Oct 16.
Blastocystis sp. is known for years as a highly prevalent anaerobic eukaryotic parasite of humans and animals. Several monophyletic clades have been delineated based on molecular data, and the occurrence of each subtype in humans and/or animal hosts has been documented. The genome of several representatives has been sequenced revealing specific traits such as an intriguing 3'-end processing of primary transcripts. Here, a first high-throughput proteomics dataset acquired on this difficult-to-cultivate parasite is presented for the zoonotic subtype T4 isolate WR1. Amongst the 2766 detected proteins, we highlighted the role of a small ADP ribosylation factor GTP-binding protein involved in intracellular traffic as major regulator of vesicle biogenesis and a voltage-dependent anion-selective channel protein because both were unexpectedly highly abundant. We show how these data may be used for gaining proteogenomics insights into Blastocystis sp. specific molecular mechanisms. We evidenced for the first time by proteogenomics a functional termination codon derived from transcript polyadenylation for seven different key cellular components.
多年来，芽囊原虫被认为是一种高度流行的人类和动物厌氧真核寄生虫。基于分子数据已经划定了几个单系进化枝，并且已经记录了每种亚型在人类和/或动物宿主中的发生情况。已经对几种代表的基因组进行了测序，揭示了特定的特征，例如引人注目的初级转录物 3'端加工。在这里，首次为动物源亚型 T4 分离株 WR1 提供了有关这种难以培养的寄生虫的高通量蛋白质组学数据集。在检测到的 2766 种蛋白质中，我们强调了参与细胞内运输的小 ADP 核糖基化因子 GTP 结合蛋白的作用，因为它是囊泡生物发生的主要调节剂，以及电压依赖性阴离子选择性通道蛋白的作用，因为这两种蛋白出乎意料地非常丰富。我们展示了如何利用这些数据深入了解芽囊原虫特有的分子机制。我们首次通过蛋白质基因组学证明了七个不同关键细胞成分的转录物多聚腺苷酸化衍生的功能终止密码子。
