用于纯化化学合成的ATP类似物ApppI的半制备型高效逆流色谱法
Semi-preparative high-performance countercurrent chromatography method for the purification of chemically synthesized ATP analogue, ApppI.
作者信息
Puljula Elina, Turhanen Petri A
机构信息
Instrument Centre, Department of Chemistry, University of Turku, Vatselankatu 2, FI-20014, Turku, Finland; School of Pharmacy, University of Eastern Finland, Biocenter Kuopio, P.O. Box 1627, FI-70211 Kuopio, Finland.
School of Pharmacy, University of Eastern Finland, Biocenter Kuopio, P.O. Box 1627, FI-70211 Kuopio, Finland.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:180-182. doi: 10.1016/j.jchromb.2017.08.038. Epub 2017 Aug 30.
An efficient high-performance countercurrent chromatography (HPCCC) based method has been developed for the purification of chemically synthesized 1-adenosin-5'-yl 3-(3-methylbut-3-enyl)triphosphoric acid diester (ApppI). ApppI is an adenosine triphosphate (ATP) analogue with biological significance due to its varied actions in the body. ApppI was synthesized and purified as its tetrabutylammonium (TBA) salt and converted successfully into its more practical sodium salt form after purification. The amount of TBA hydroxide (2.0, 2.5 and 3.0 eq) used in the synthesis of ApppI was shown to exert an effect on the purification process with HPCCC and on the overall yield (8%, 16% and 22%, respectively). 1-Adenosin-5'-yl 3-(3-methylbut-3-enyl)diphosphoric acid diester (AppI) was also isolated as a side product.
已开发出一种基于高效高性能逆流色谱（HPCCC）的方法，用于纯化化学合成的1-腺苷-5'-基 3-(3-甲基丁-3-烯基)三磷酸二酯（ApppI）。ApppI是一种三磷酸腺苷（ATP）类似物，因其在体内的多种作用而具有生物学意义。ApppI以其四丁基铵（TBA）盐的形式合成并纯化，纯化后成功转化为更实用的钠盐形式。结果表明，在ApppI合成中使用的氢氧化四丁基铵的量（2.0、2.5和3.0当量）对HPCCC的纯化过程和总产率（分别为8%、16%和22%）有影响。1-腺苷-5'-基 3-(3-甲基丁-3-烯基)二磷酸二酯（AppI）也作为副产物被分离出来。
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