Jung Gi Hoon, Lim Eun Seob, Woo Min-Ah, Lee Joo Young, Kim Joo-Sung, Paik Hyun-Dong
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
J Microbiol Biotechnol. 2017 Nov 28;27(11):1942-1951. doi: 10.4014/jmb.1703.03052.
and are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient strains versus biofilm-forming strains isolated from chickens, to understand the relationship between extracellular DNase activity and biofilm formation. A biofilm-forming reference strain, NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants. The biofilm non-forming strains or supernatants significantly prohibited the biofilm formation of NCTC11168. In addition, the strains degraded pre-formed biofilms of NCTC11168. Degradation of NCTC11168 biofilm was confirmed after treatment with the supernatant of the biofilm non-forming strain 2-1 by confocal laser scanning microscopy. Quantitative analysis of the biofilm matrix revealed reduction of extracellular DNA (16%) and proteins (8.7%) after treatment. Whereas the biofilm-forming strains Y23-5 and 34-3 isolated from raw chickens and the NCTC11168 reference strain showed no extracellular DNase activity against their own genomic DNA, most biofilm non-forming strains tested, including 2-1, 34-1, and 63-1, exhibited obvious extracellular DNase activities against their own or 11168 genomic DNA, except for one biofilm non-former, 22-1. Our results suggest that extracellular DNase activity is a common feature suppressing biofilm formation among biofilm non-forming or strains of chicken origin.
[细菌名称1]和[细菌名称2]是重要的食源性病原体，在禽肉中尤为常见。在本研究中，对从鸡中分离出的生物膜缺陷型[细菌名称1]菌株与生物膜形成型[细菌名称1]菌株的细胞外DNase活性进行了研究，以了解细胞外DNase活性与生物膜形成之间的关系。将生物膜形成参考菌株[细菌名称3] NCTC11168与从生鸡中分离出的非生物膜形成菌株或其上清液共同孵育。非生物膜形成菌株或上清液显著抑制了NCTC11168的生物膜形成。此外，这些菌株降解了NCTC11168预先形成的生物膜。通过共聚焦激光扫描显微镜观察发现，用非生物膜形成菌株2-1的上清液处理后，NCTC11168生物膜被降解。生物膜基质的定量分析显示，处理后细胞外DNA减少了16%，蛋白质减少了8.7%。从生鸡中分离出的生物膜形成菌株Y23-5和34-3以及参考菌株NCTC11168对其自身基因组DNA没有细胞外DNase活性，而大多数测试的非生物膜形成菌株，包括2-1、34-1和63-1，对其自身或11168基因组DNA表现出明显的细胞外DNase活性，但有一个非生物膜形成菌株22-1除外。我们的结果表明，细胞外DNase活性是鸡源非生物膜形成型[细菌名称1]或[细菌名称2]菌株中抑制生物膜形成的一个共同特征。
