Tang Xin, Shi Xiaobin, Zhang Deyong, Li Fan, Yan Fei, Zhang Youjun, Liu Yong, Zhou Xuguo
College of Plant Protection, Hunan Agricultural University, Changsha, 410125, China.
Hunan Academy of Agricultural Science, Hunan Plant Protection Institute, Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, No. 726, Yuanda Road, Furong District, Hunan province, Changsha, 410125, China.
Virol J. 2017 Sep 4;14(1):169. doi: 10.1186/s12985-017-0833-2.
In recent years, two of the crinivirus, Tomato chlorosis virus (ToCV) and Cucurbit chlorotic yellows virus (CCYV) have gained increasing attention due to their rapid spread and devastating impacts on vegetable production worldwide. Both of these viruses are transmitted by the sweet potato whitefly, Bemisia tabaci (Gennadius), in a semi-persistent manner. Up to now, there is still lack of report in Hainan, the south of China.
We used observational and experimental methods to explore the prevalence and incidence dynamic of CCYV and ToCV transmitted by whiteflies in Hainan of China.
In 2016, the chlorosis symptom was observed in the tomato and cucumber plants with a large number of B. tabaci on the infected leaves in Hainan, China, with the incidence rate of 69.8% and 62.6% on tomato and cucumber, respectively. Based on molecular identification, Q biotype was determined with a viruliferous rate of 65.0% and 55.0% on the tomato and cucumber plants, respectively. The weed, Alternanthera philoxeroides near the tomato and cucumber was co-infected by the two viruses. Furthermore, incidence dynamic of ToCV and CCYV showed a close relationship with the weed, Alternanthera philoxeroides, which is widely distributed in Hainan.
Our results firstly reveal that the weed, A. philoxeroides is infected by both ToCV and CCYV. Besides, whiteflies showed a high viruliferous rate of ToCV and CCYV. Hainan is an extremely important vegetable production and seed breeding center in China. If the whitefly can carry these two viruses concurrently, co-infection in their mutual host plants can lead to devastating losses in the near future.
近年来，两种曲顶病毒，番茄褪绿病毒（ToCV）和葫芦褪绿黄化病毒（CCYV）因其迅速传播并对全球蔬菜生产造成毁灭性影响而日益受到关注。这两种病毒均由烟粉虱（Bemisia tabaci (Gennadius)）以半持久性方式传播。截至目前，中国南部的海南仍缺乏相关报道。
我们采用观察和实验方法，探究在中国海南由粉虱传播的CCYV和ToCV的流行情况及发病动态。
2016年，在中国海南，番茄和黄瓜植株出现褪绿症状，受感染叶片上有大量烟粉虱，番茄和黄瓜的发病率分别为69.8%和62.6%。基于分子鉴定，确定为Q型生物型，番茄和黄瓜植株上带毒率分别为65.0%和55.0%。番茄和黄瓜附近的杂草空心莲子草同时感染了这两种病毒。此外，ToCV和CCYV的发病动态与在海南广泛分布的杂草空心莲子草密切相关。
我们的结果首次揭示杂草空心莲子草同时感染了ToCV和CCYV。此外，粉虱对ToCV和CCYV的带毒率很高。海南是中国极其重要的蔬菜生产和种子繁育中心。如果粉虱能同时携带这两种病毒，它们在共同寄主植物上的共同感染可能在不久的将来导致毁灭性损失。
