Oyanagi K, Kuniya Y, Nagao M, Tsuchiyama A, Nakao T
Tohoku J Exp Med. 1987 May;152(1):47-51. doi: 10.1620/tjem.152.47.
The cytotoxicities of sodium benzoate was studied using primary culture of hepatocytes established from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in serum-free culture medium. The activities of ornithine transcarbamylase (as a marker of mitochondria) and tyrosine aminotransferase (as a marker of cytosol) were clearly suppressed by sodium benzoate at concentration in excess of 500 micrograms/ml. Intracellular protein synthesis and DNA synthesis were also suppressed, and the suppression of DNA synthesis was observed even with a lower concentration of benzoate (100 micrograms/ml).
采用胶原酶灌注技术从成年大鼠肝脏建立原代肝细胞培养物，并在无血清培养基中维持单层培养，研究了苯甲酸钠的细胞毒性。当苯甲酸钠浓度超过500微克/毫升时，鸟氨酸转氨甲酰酶（作为线粒体的标志物）和酪氨酸转氨酶（作为胞质溶胶的标志物）的活性明显受到抑制。细胞内蛋白质合成和DNA合成也受到抑制，甚至在较低浓度的苯甲酸钠（100微克/毫升）下也观察到DNA合成受到抑制。
