癌症干细胞相关mRNA的表达作为肝细胞癌的预后标志物

Expression of Cancer Stem Cell-associated mRNA Serves as Prognostic Marker for Hepatocellular Carcinoma.

作者信息

Sakabe Tomohiko, Azumi Junya, Umekita Yoshihisa, Toriguchi Kan, Hatano Etsuro, Hirooka Yasuaki, Shiota Goshi

机构信息

Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago, Japan.

Division of Organ Pathology, Department of Pathology, Faculty of Medicine, Tottori University, Yonago, Japan.

出版信息

Anticancer Res. 2017 Sep;37(9):4881-4888. doi: 10.21873/anticanres.11897.

DOI:10.21873/anticanres.11897

PMID:28870909

Abstract

BACKGROUND/AIM: Cancer stem cells (CSCs) are associated with prognosis of hepatocellular carcinoma (HCC). In our previous study, we created cDNA microarray databases on the CSC population of human HuH7 cells. In the present study, we identified genes that might serve as prognostic markers of HCC by employing existing databases.

MATERIALS AND METHODS

Expressions of glutathione S-transferase pi 1 (GSTP1), lysozyme (LYZ), C-X-C motif chemokine ligand 5 (CXCL5), interleukin-8 (IL8) and dickkopf WNT signaling pathway inhibitor 1 (DKK1), the five most highly expressed genes in the CSC cDNA microarray databases, were examined in 99 patients with HCC by real-time polymerase chain reaction (qRT-PCR), and their clinical significance was analyzed.

RESULTS

The Kaplan-Meier analysis showed that both overall and cancer-specific survival were significantly longer in patients with low DKK1 expression than in those with high DKK1 expression. The multivariate analysis revealed that overall survival was negatively associated with albumin and positively associated with alkaline phosphatase (ALP), serosal invasion and stage, and cancer-specific survival was positively associated with ALP, portal vein invasion and DKK1 mRNA.

CONCLUSION

Expression of CSC-associated DKK1 mRNA might be an unfavorable prognostic marker for patients with HCC.

摘要

背景/目的：癌症干细胞（CSCs）与肝细胞癌（HCC）的预后相关。在我们之前的研究中，我们创建了关于人HuH7细胞CSC群体的cDNA微阵列数据库。在本研究中，我们通过利用现有数据库鉴定了可能作为HCC预后标志物的基因。

材料与方法

通过实时聚合酶链反应（qRT-PCR）检测了CSC cDNA微阵列数据库中表达最高的五个基因，即谷胱甘肽S-转移酶pi 1（GSTP1）、溶菌酶（LYZ）、C-X-C基序趋化因子配体5（CXCL5）、白细胞介素-8（IL8）和Dickkopf WNT信号通路抑制剂1（DKK1）在99例HCC患者中的表达情况，并分析了其临床意义。