Pasaribu Buntora, Wang Miki M C, Jiang Pei-Luen
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, 402, Taiwan.
Department of Biotechnology, National Formosa University, No. 64, Wunhua Rd., Huwei Township, Yunlin County, 632, Taiwan.
Biotechnol Lett. 2017 Nov;39(11):1757-1763. doi: 10.1007/s10529-017-2414-2. Epub 2017 Sep 4.
To investigate the oil body protein and function in seeds of mature seagrass, Thalassia hemprichii.
Seeds of mature seagrass T. hemprichii when stained with a fluorescent probe BODIPY showed the presence of oil bodies in intracellular cells. Triacylglycerol was the major lipid class in the seeds. Protein extracted from seagrass seeds was subjected to immunological cross-recognition with land plant seed oil body proteins, such as oleosin and caleosin, resulting in no cross-reactivity. An oleosin-like gene was found in seagrass seeds. Next generation sequencing and sequence alignment indicated that the deduced seagrass seed oleosin-like protein has a central hydrophobic domain responsible for their anchoring onto the surface of oil bodies. Phylogenetic analysis showed that the oleosin-like protein was evolutionarily closer to pollen oleosin than to seed oleosins.
Oil body protein found in seagrass seeds represent a distinct class of land seed oil body proteins.
研究成熟海草泰来草种子中的油体蛋白及其功能。
用荧光探针BODIPY对成熟海草泰来草种子进行染色时，细胞内可见油体。三酰甘油是种子中的主要脂质类别。从海草种子中提取的蛋白质与陆地植物种子油体蛋白（如油质蛋白和钙结合油体蛋白）进行免疫交叉识别，未产生交叉反应。在海草种子中发现了一个类似油质蛋白的基因。新一代测序和序列比对表明，推导的海草种子类油质蛋白具有一个中央疏水结构域，负责将其锚定在油体表面。系统发育分析表明，类油质蛋白在进化上与花粉油质蛋白比与种子油质蛋白更接近。
海草种子中发现的油体蛋白代表了一类与陆地种子油体蛋白不同的蛋白。
