Department of Plant Biology & Pathology, Rutgers University , New Brunswick, USA.
Department of Biotechnology, National Formosa University , Yunlin, Taiwan.
Plant Signal Behav. 2020 Aug 2;15(8):1779486. doi: 10.1080/15592324.2020.1779486. Epub 2020 Jun 17.
Oil bodies are essential energy storage organelles that are generally present in the seeds of plants. Caleosin protein has been identified in the seed oil bodies of seed. In this study, mature pollen grains were collected from cycad elliptical cones. However, the isolation and identification of oil bodies protein from mature grains have never been experimentally determined. Ultrastructural studies have shown that the oil bodies were present in pollen grains. Lipid analysis showed that oil bodies are predominantly composed of triacylglycerol. Complete cDNA fragments encoding the caleosin were obtained by PCR cloning. Phylogenetic tree analyzes showed that cycad pollen caleosin is closely related to the caleosin of seeds. Fresh pollen grains were allowed to germinate for 48 h in a germination medium containing 2% sucrose and 0.01% HBO Pollen germination were recorded periodically from day one to day 10 and the results showed that the pollen elongate the tube and increasing of triacylglycerol(TAG) after 4 days.
油体是植物种子中普遍存在的重要储能细胞器。在种子的油体中已经鉴定出钙蛋白。在这项研究中，从苏铁椭圆形锥体中收集成熟花粉粒。然而，从未从成熟花粉粒中分离和鉴定油体蛋白。超微结构研究表明，油体存在于花粉粒中。脂质分析表明，油体主要由三酰基甘油组成。通过 PCR 克隆获得了编码钙蛋白的完整 cDNA 片段。系统发育树分析表明，苏铁花粉钙蛋白与种子中的钙蛋白密切相关。新鲜花粉粒在含有 2%蔗糖和 0.01% HBO 的萌发培养基中允许萌发 48 小时。从第一天到第十天定期记录花粉萌发情况，结果表明花粉在第 4 天伸长管并增加三酰基甘油(TAG)。
