Ginsberg B H, Brown T, Raizada M
Diabetes. 1979 Sep;28(9):823-7. doi: 10.2337/diab.28.9.823.
The Friend erythroleukemia cell has an insulin receptor with all the properties of mammalian insulin receptors: rapid, reversible, and saturable binding of insulin; specific for insulin and insulin analogs; inversely proportional to temperatures; sharply pH dependent (optimum = 8.0); and demonstrated ligand-induced accelerated dissociation consistent with negative cooperativity. There were 17,200 sites per cell. After induction by dimethylsulfoxide, 80% of the cells became benzidine positive (i.e., contained hemoglobin). The receptor concentration dropped to 4300 sites per cell, while the remaining receptors retained all the initial binding characteristics. This loss of receptors could not be attributed directly to either dimethylsulfoxide or changes in cell size. Thus, during the process of differentiation, the concentration of insulin receptors in the Friend erythroleukemia cell decreases.
胰岛素的快速、可逆和饱和结合；对胰岛素及胰岛素类似物具有特异性；与温度成反比；对pH值变化敏感（最适pH = 8.0）；且显示出与负协同性一致的配体诱导加速解离。每个细胞有17200个受体位点。经二甲基亚砜诱导后，80%的细胞呈联苯胺阳性（即含有血红蛋白）。受体浓度降至每个细胞4300个位点，而其余受体保留了所有初始结合特性。这种受体的减少不能直接归因于二甲基亚砜或细胞大小的变化。因此，在分化过程中，弗氏红白血病细胞中胰岛素受体的浓度降低。
