Kasahara M, Hinkle P C, Ikawa Y, Amanuma H
Biochim Biophys Acta. 1986 Apr 25;856(3):615-23. doi: 10.1016/0005-2736(86)90155-0.
A transport system for D-glucose was found in a Friend erythroleukemia cell line, T-3-C1-2-O and was characterized as a facilitated diffusion system. D-Glucose transport activity showed a half-saturation concentration of 2.2 mM and was inhibited by mercuric ions, cytochalasin B, phloretin, and stilbestrol, but was not strongly inhibited by phloridzin. Transport of 3-O-methyl-D-glucose was faster than D-glucose and the intracellular concentration of the sugar was found to reach the concentration in the assay medium. The treatment of cells with a differentiation-inducing reagent, dimethylsulfoxide(Me2SO), for 24 h caused a marked decrease in glucose transport activity due to a decrease in Vmax. In an induction-insensitive Friend cell line, T-3-K-1, D-glucose transport activity was low in untreated cells and Me2SO treatment did not cause a significant decrease in transport activity. The results obtained in this study indicate that the decrease in glucose transport activity is not due to the direct effect of Me2SO on transport activity, but is associated with the induction of differentiation. By immunoblotting cell lysates of T-3-C1-2-O cells using antibody to human erythrocyte glucose transporter, a single major band having a molecular weight of 52,000 was detected, which may be a glucose transporter in Friend cells.
在Friend红白血病细胞系T-3-C1-2-O中发现了一种D-葡萄糖转运系统，其特征为易化扩散系统。D-葡萄糖转运活性的半饱和浓度为2.2 mM，且受到汞离子、细胞松弛素B、根皮素和己烯雌酚的抑制，但未受到根皮苷的强烈抑制。3-O-甲基-D-葡萄糖的转运速度比D-葡萄糖快，且发现该糖在细胞内的浓度能达到测定培养基中的浓度。用分化诱导剂二甲基亚砜（Me2SO）处理细胞24小时，由于Vmax降低，导致葡萄糖转运活性显著下降。在对诱导不敏感的Friend细胞系T-3-K-1中，未处理细胞的D-葡萄糖转运活性较低，Me2SO处理并未导致转运活性显著下降。本研究所得结果表明，葡萄糖转运活性的下降并非由于Me2SO对转运活性的直接作用，而是与分化诱导相关。使用抗人红细胞葡萄糖转运蛋白抗体对T-3-C1-2-O细胞裂解物进行免疫印迹分析，检测到一条分子量为52,000的单一主要条带，其可能是Friend细胞中的葡萄糖转运蛋白。
