某些酚类抗氧化剂对脑突触体摄取Ca2+及神经递质释放的抑制作用。

Inhibition by some phenolic antioxidants of Ca2+ uptake and neurotransmitter release from brain synaptosomes.

作者信息

Zoccarato F, Pandolfo M, Deana R, Alexandre A

出版信息

Biochem Biophys Res Commun. 1987 Jul 31;146(2):603-10. doi: 10.1016/0006-291x(87)90571-7.

Abstract

We present observations on the action of the phenolic antioxidants 2,3-tert-butyl-4-methoxyphenol (BHT); 2,6-di-tert-butyl-4-methylphenol (BHA); n-propyl-gallate and nordihydroguaiaretic acid (NDGA) on the function of guinea pig cerebral cortex synaptosomes. While the Ca2+ uptake observed under non depolarizing conditions is not affected by these agents, the depolarization induced Ca2+ uptake is strongly inhibited. Similarly the phenolic antioxidants studied inhibit the Ca2+ plus depolarization induced exocytosis of GABA and glutamate. These results which are similar to those previously obtained in blood platelets (Alexandre, A. et. al. Biochem. Biophys. Res. Comm. 139, 509-514, 1986), may indicate that free radical intermediates are involved in stimulus-secretion coupling.

摘要

我们展示了关于酚类抗氧化剂2,3-二叔丁基-4-甲氧基苯酚（BHT）、2,6-二叔丁基-4-甲基苯酚（BHA）、没食子酸正丙酯和去甲二氢愈创木酸（NDGA）对豚鼠大脑皮质突触体功能作用的观察结果。虽然在非去极化条件下观察到的Ca2+摄取不受这些药剂影响，但去极化诱导的Ca2+摄取受到强烈抑制。同样，所研究的酚类抗氧化剂抑制Ca2+加去极化诱导的GABA和谷氨酸的胞吐作用。这些结果与先前在血小板中获得的结果相似（亚历山大，A.等人，《生物化学与生物物理学研究通讯》139，509 - 514，1986），可能表明自由基中间体参与了刺激 - 分泌偶联。