Zhang Yu-Xin, Li Cong-Yin, Liu Chuan, Xu Jiang, Xiang Li, Fan Gang, Zhang Yi
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):572-577. doi: 10.4268/cjcmm20160404.
DNA barcoding technique in combination with UFLC analysis technology was used to evaluate the quality of Tibetan medicine Pterocephalus hookeri from species identification and chemical qualitative and other aspects. Hybrid identification was established by DNA barcoding; UFLC-PDA was adopted to analyse fingerprint of different parts of Pterocephali Herba, and SPSS and Grey relation software were used for data analysis. The result showed that DNA barcoding is an accurate and reliable method in origin identification of Pterocephalus hookeri. The compounds in overground is more than underground by analysis of the different part fingerprint by UFLC. The genetic gene may be involved in the secondary metabolites of iridoid glycosides. Pertinence between gene and chemical component, as a new model established, could be suited for quality evaluation and resources protection.
采用DNA条形码技术结合超高效液相色谱（UFLC）分析技术，从物种鉴定、化学定性等方面对藏药翼首草的质量进行评价。通过DNA条形码建立杂种鉴定；采用UFLC-PDA分析翼首草不同部位的指纹图谱，并运用SPSS和灰色关联软件进行数据分析。结果表明，DNA条形码技术是翼首草基原鉴定的准确可靠方法。通过UFLC分析不同部位指纹图谱可知，地上部分的化合物比地下部分多。遗传基因可能参与环烯醚萜苷类次生代谢产物的合成。所建立的基因与化学成分相关性新模型，可适用于质量评价和资源保护。
