Wang Wen-Xiang, Su Jin-Song, Yu Meng, Liu Xiu-Hua, Yang Hong-Jun, Zou Zhong-Mei, Zhang Yi, Tang Ce
Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
Zhongguo Zhong Yao Za Zhi. 2019 Aug;44(15):3170-3177. doi: 10.19540/j.cnki.cjcmm.20190628.204.
The intestinal absorption characteristics of ten iridoid glycosides and phenolic acids in the Pterocephali Herba were evaluated via rat intestinal valgus model. The intestinal sac fluids at different time after administration of high,medium and low concentrations of Pterocephali Herba extract were collected and ten chemical components in fluid samples were detected by UPLC-PDA. Accumulative absorbed doses( Q) and absorption rate constants( Ka) of ten chemical constituents were calculated,while proportions between Pterocephali Herba extract and intestinal absorption liquid were compared. The results showed that the intestinal absorption of 10 chemical components was linear absorption( R2>0. 9) at different concentrations,which accorded with the zero-order absorption rate. The absorption rate constant was related to the concentration of the drug and the intestinal site,which indicated that intestinal adsorption mechanism of the components were passive diffusion and active transport. Proportions of chemical constituents in intestinal sac fluid were different from those in Pterocephali Herba extract. Therefore,those ten chemical components in Pterocephali Herba extract can be absorbed in whole intestine. Everted intestinal sac model can be used to evaluate intestinal absorption characteristics of ingredients in Pterocephali Herba extract effectively.
通过大鼠肠外翻模型评价了翼首草中10种环烯醚萜苷和酚酸的肠道吸收特性。收集高、中、低浓度翼首草提取物给药后不同时间的肠囊液，采用超高效液相色谱-光电二极管阵列检测器（UPLC-PDA）检测囊液中的10种化学成分。计算10种化学成分的累积吸收量（Q）和吸收速率常数（Ka），并比较翼首草提取物与肠道吸收液之间的比例。结果表明，10种化学成分在不同浓度下的肠道吸收均为线性吸收（R2>0.9），符合零级吸收速率。吸收速率常数与药物浓度和肠道部位有关，表明各成分的肠道吸附机制为被动扩散和主动转运。肠囊液中化学成分比例与翼首草提取物不同。因此，翼首草提取物中的这10种化学成分可在整个肠道被吸收。肠外翻模型可有效用于评价翼首草提取物中成分的肠道吸收特性。
