Liu Yue, Liu Chuan, Tan Er, Fan Gang, Xiang Li, Li Xiao-Dong, Zhang Yi
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):578-585. doi: 10.4268/cjcmm20160405.
To differentiate three medicinal Hippopahe species of seabuckthorn, a combined genetic and chemical identification method was established in this study. ITS2 and psbA-trnH were tested for identification of 3 species of seabuckthorn. Detection of the kimura 2-parameter (K2P) distance, the neighbor-joining (NJ) tree and the barcoding gap were used to assess the identification efficiency. ¹H-NMR based metabolic method was applied to acquire the profile of metabolites. PCA was used to analysis the metabolite data. The results indicated that DNA barcode combined ¹H-NMR based metabolic method is a powerful tool for the identification of 3 medicinal Hippopahe species of seabuckthorn. The finding demonstrated that different genetic variation and chemical constituents existed among 3 medicinal Hippopahe species of seabuckthorn. The combined identification method will improve the reliability of species discrimination and could be applicable to much other ethnic medicine which has various origins in China.
为区分三种药用沙棘属植物，本研究建立了一种基因与化学相结合的鉴定方法。对ITS2和psbA-trnH进行检测以鉴定3种沙棘属植物。采用检测Kimura 2-参数（K2P）距离、邻接法（NJ）树和条形码间隙来评估鉴定效率。应用基于¹H-NMR的代谢方法获取代谢物谱。主成分分析（PCA）用于分析代谢物数据。结果表明，DNA条形码结合基于¹H-NMR的代谢方法是鉴定3种药用沙棘属植物的有力工具。研究结果表明，3种药用沙棘属植物之间存在不同的遗传变异和化学成分。这种联合鉴定方法将提高物种鉴别的可靠性，并可应用于中国其他多种来源的民族药物。
