[长期培养过程中胚胎肝脏造血细胞的保护潜力]

[Protective potential of hematopoietic cells of the embryonic liver during long-term culture].

作者信息

Samolina N L, Udalov G A

出版信息

Biull Eksp Biol Med. 1987 Jul;104(7):93-6.

Abstract

The influence of the duration of the organ cultivation on the protective potential of mouse fetal liver hemopoietic cells was investigated. The protective potential was evaluated according to a 3-week viability of the lethally irradiated (mean LD88/21) mice following syngeneic transplantation. During 20 days of cultivation the protective potential (calculated per number of injected cells) remained at the initial level. With more prolonged cultivation (24-62 days) the protective potential was retained only in part of cultures and the mean effect was reduced. No parallelism has been revealed between the totipotent hemopoietic precursors capable of replacing the hemopoietic cells of an irradiated recipient and the CFUs in long-cultivated fetal liver cultures.

摘要

研究了器官培养持续时间对小鼠胎肝造血细胞保护潜能的影响。根据同基因移植后致死性照射（平均LD88/21）小鼠的3周存活率评估保护潜能。在培养的20天内，保护潜能（按注射细胞数计算）保持在初始水平。随着培养时间延长（24 - 62天），保护潜能仅在部分培养物中得以保留，且平均效应降低。在能够替代受照射受体造血细胞的全能造血前体细胞与长期培养的胎肝培养物中的集落形成单位之间未发现平行关系。

相似文献

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[Protective potential of hematopoietic cells of the embryonic liver during long-term culture].[长期培养过程中胚胎肝脏造血细胞的保护潜力]

Biull Eksp Biol Med. 1987 Jul;104(7):93-6.

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[Semisyngeneic transplantation of hematopoietic cells of native and cultured embryonic liver].[天然与培养的胚胎肝脏造血细胞的半同基因移植]

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Ex vivo culture rescues hematopoietic stem cells with long-term repopulating capacity following harvest from lethally irradiated mice.体外培养可挽救从经致死剂量照射的小鼠体内采集的具有长期重建能力的造血干细胞。

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