Samoĭlina N L
Biull Eksp Biol Med. 1988 Feb;105(2):228-31.
The protective ability and graft-versus-host (GVH) activity in parental strain hematopoietic fetal liver cells (FLC) transplanted to irradiated F1 hybrids were evaluated quantitatively. A 21-day survival of more than 80% of semi-syngeneic mouse recipients required the injection of 2-5 X 10(6) nucleated fetal liver cells (FLC). The same effect could be obtained with FLC cultivated for 4-15 days. 5 to 25 X 10(6) parental FLC were necessary to induce a considerable GVH mortality within 2-3 months after transplantation. Thus, the minimal cell doses of both native and cultivated FLC enough for the maximal protective effect have proved ineffective for the provocation of the fetal GVH disease. Hematopoietic cells from long-term FLC cultures had a low protective potential though they could contain high CFUs concentration. This discrepancy shows clearly that such polypotent precursors as CFUs have no ability to restore hematopoiesis, in other words they cannot be totipotent stem cells.
对移植到经辐射的F1杂种体内的亲代品系造血胎肝细胞（FLC）的保护能力和移植物抗宿主（GVH）活性进行了定量评估。超过80%的半同基因小鼠受体存活21天需要注射2 - 5×10⁶个有核胎肝细胞（FLC）。培养4 - 15天的FLC也能获得相同效果。移植后2 - 3个月内，诱导相当程度的GVH死亡率需要5至25×10⁶个亲代FLC。因此，已证明足以产生最大保护作用的天然和培养FLC的最小细胞剂量，对引发胎儿GVH病无效。长期FLC培养物中的造血细胞虽然可能含有高浓度的集落形成单位（CFU），但其保护潜力较低。这种差异清楚地表明，像CFU这样的多能前体细胞没有恢复造血的能力，换句话说，它们不可能是全能干细胞。
