视网膜细胞培养中的血管活性肠多肽系统：免疫细胞化学与生理学

A vasoactive intestinal polypeptide system in retinal cell cultures: immunocytochemistry and physiology.

作者信息

Fukuda M, Yeh H H, Puro D G

出版信息

Brain Res. 1987 Jun 23;414(1):177-81. doi: 10.1016/0006-8993(87)91343-6.

Abstract

The purpose of this study was to search for vasoactive intestinal polypeptide (VIP)-like immunoreactivity and VIP-mediated effects in cultures containing cells from the mammalian retina. VIP-like immunoreactivity was detected by indirect immunocytochemistry within 6 days after plating dissociated retinal cells from embryonic day-19 rats. In electrophysiological experiments, VIP was found to facilitate evoked transmission at cholinergic synapses formed by retinal neurons in culture.

摘要

本研究的目的是在含有哺乳动物视网膜细胞的培养物中寻找血管活性肠肽（VIP）样免疫反应性及VIP介导的效应。通过间接免疫细胞化学方法，在接种来自胚胎19天大鼠的视网膜解离细胞后6天内检测到了VIP样免疫反应性。在电生理实验中，发现VIP可促进培养的视网膜神经元形成的胆碱能突触处的诱发传递。