Department of Neurobiology, Stanford University, Stanford, United States.
Elife. 2017 Sep 5;6:e29222. doi: 10.7554/eLife.29222.
Eye movements provide insights about a wide range of brain functions, from sensorimotor integration to cognition; hence, the measurement of eye movements is an important tool in neuroscience research. We describe a method, based on magnetic sensing, for measuring eye movements in head-fixed and freely moving mice. A small magnet was surgically implanted on the eye, and changes in the magnet angle as the eye rotated were detected by a magnetic field sensor. Systematic testing demonstrated high resolution measurements of eye position of <0.1°. Magnetic eye tracking offers several advantages over the well-established eye coil and video-oculography methods. Most notably, it provides the first method for reliable, high-resolution measurement of eye movements in freely moving mice, revealing increased eye movements and altered binocular coordination compared to head-fixed mice. Overall, magnetic eye tracking provides a lightweight, inexpensive, easily implemented, and high-resolution method suitable for a wide range of applications.
眼球运动提供了广泛的大脑功能的见解，从感觉运动整合到认知；因此，眼球运动的测量是神经科学研究中的重要工具。我们描述了一种基于磁传感的方法，用于测量头部固定和自由移动的小鼠的眼球运动。在眼球上进行手术植入一个小磁铁，通过磁场传感器检测磁铁角度的变化，以检测眼球的转动。系统测试表明，眼球位置的分辨率测量精度可以达到 <0.1°。与成熟的眼线圈和视频眼动记录方法相比，磁性眼球追踪具有几个优势。最显著的是，它为自由移动的小鼠提供了可靠的、高分辨率的眼球运动测量方法，与头部固定的小鼠相比，该方法揭示了眼球运动的增加和双眼协调的改变。总的来说，磁性眼球追踪提供了一种轻便、廉价、易于实现且具有高分辨率的方法，适用于广泛的应用。
