Stahl J S, van Alphen A M, De Zeeuw C I
Department of Neurology, Case Western Reserve University, 11100 Euclid Avenue, 44106-5040, Cleveland, OH, USA.
J Neurosci Methods. 2000 Jun 30;99(1-2):101-10. doi: 10.1016/s0165-0270(00)00218-1.
Interest in connecting molecular biology and behavior is motivating research into the eye movements of mice. Unfortunately, recording eye movements in this diminutive animal is technically difficult. We present the first method for obtaining calibrated video oculography, and contrast the results with simultaneously obtained scleral search coil recordings in C57BL/6 pigmented mice. We determined the distance of the pupil from the center of corneal curvature, based upon relative motions of the pupil and corneal reflections during camera movements, and used the distance to convert subsequent video measurements of pupil motion to eye rotation. We recorded responses during sinusoidal rotation (0.1-1.6 Hz) in the light, by video prior to search coil implantation, and by video and search coil simultaneously following implantation. Pre-implantation, video-derived gains ranged from 0.86+/-0.03 (mean+/-SD) at 0.1 Hz to 0.95+/-0.03 at 0.8 Hz. Phase progressed monotonically from -3.1+/-2. 6 degrees (eye leads head) at 0.1 Hz to +5.9+/-1.1 degrees at 1.6 Hz. Coil implantation reduced the range of video-derived gains to 0. 64-0.79. This reduction reflects disruption of normal behavior by the coil. Coil data confirmed the video results. Video and search coil techniques each have advantages. Specific precautions are required when designing and interpreting experiments using the coil technique.
将分子生物学与行为联系起来的兴趣正推动着对小鼠眼球运动的研究。不幸的是，在这种小型动物中记录眼球运动在技术上具有挑战性。我们提出了第一种获得校准视频眼动描记术的方法，并将结果与在C57BL/6有色小鼠中同时获得的巩膜搜索线圈记录进行对比。我们根据相机移动过程中瞳孔与角膜反射的相对运动确定了瞳孔到角膜曲率中心的距离，并使用该距离将随后视频测量的瞳孔运动转换为眼球旋转。我们在光照下正弦旋转（0.1 - 1.6 Hz）期间进行记录，植入搜索线圈前通过视频记录，植入后通过视频和搜索线圈同时记录。植入前，视频得出的增益在0.1 Hz时为0.86±0.03（平均值±标准差），在0.8 Hz时为0.95±0.03。相位从0.1 Hz时的 - 3.1±2.6度（眼球超前头部）单调进展到1.6 Hz时的 + 5.9±1.1度。线圈植入使视频得出的增益范围缩小到0.64 - 0.79。这种缩小反映了线圈对正常行为的干扰。线圈数据证实了视频结果。视频和搜索线圈技术各有优势。在设计和解释使用线圈技术的实验时需要采取特定的预防措施。
