Vaníčková Lucie, Nagy Radka, Pompeiano Antonio, Kalinová Blanka
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
Laboratory of Infochemicals, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.
PLoS One. 2017 Sep 5;12(9):e0184102. doi: 10.1371/journal.pone.0184102. eCollection 2017.
Bactrocera invadens Drew, Tsuruta & White, Bactrocera papayae Drew & Hancock, and Bactrocera philippinensis Drew & Hancock, key pest species within the Bactrocera dorsalis species complex, have been recently synonymized under the name Bactrocera dorsalis (Hendel). The closely related Bactrocera carambolae Drew & Hancock remains as a discrete taxonomic entity. Although the synonymizations have been accepted by most researchers, debate about the species limits remains. Because of the economic importance of this group of taxa, any new information available to support or deny the synonymizations is valuable. We investigated the chemical epicuticle composition of males and females of B. dorsalis, B. invadens, B. papayae, B. philippinensis, and B. carambolae by means of one- and two-dimensional gas chromatography-mass spectrometry, followed by multiple factor analyses and principal component analysis. Clear segregation of complex cuticule profiles of both B. carambolae sexes from B. dorsalis (Hendel) was observed. In addition to cuticular hydrocarbons, abundant complex mixtures of sex-specific oxygenated lipids (three fatty acids and 22 fatty acid esters) with so far unknown function were identified in epicuticle extracts from females of all species. The data obtained supports both taxonomic synonymization of B. invadens, B. papayae, and B. philippinensis with B. dorsalis, as well as the exclusion of B. carambolae from B. dorsalis.
入侵果实蝇（Bactrocera invadens Drew、Tsuruta & White）、番木瓜实蝇（Bactrocera papayae Drew & Hancock）和菲律宾实蝇（Bactrocera philippinensis Drew & Hancock）是桔小实蝇复合种内的主要害虫种类，最近已被归为桔小实蝇（Bactrocera dorsalis (Hendel)）的同物异名。亲缘关系密切的杨桃实蝇（Bactrocera carambolae Drew & Hancock）仍是一个独立的分类实体。尽管大多数研究人员已接受这些同物异名，但关于物种界限的争论仍在继续。由于这一类群分类单元具有经济重要性，任何支持或否定这些同物异名的新信息都很有价值。我们通过一维和二维气相色谱 - 质谱联用技术，对桔小实蝇、入侵果实蝇、番木瓜实蝇、菲律宾实蝇和杨桃实蝇的雌雄成虫体表化学成分进行了研究，随后进行了多因素分析和主成分分析。观察到杨桃实蝇两性复杂的表皮轮廓与桔小实蝇（Hendel）明显分离。除了表皮碳氢化合物外，在所有物种雌虫的表皮提取物中还鉴定出大量功能未知的性别特异性氧化脂质（三种脂肪酸和22种脂肪酸酯）复杂混合物。所获得的数据既支持入侵果实蝇、番木瓜实蝇和菲律宾实蝇与桔小实蝇的分类同物异名，也支持将杨桃实蝇排除在桔小实蝇之外。
