Arora Rohit, Arora Saroj, Vig Adarsh Pal
Department of Botanical & Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
Department of Botanical & Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
Food Chem. 2018 Jan 15;239:1085-1089. doi: 10.1016/j.foodchem.2017.07.059. Epub 2017 Jul 13.
In the present study reverse-phase UHPLC-PDA technique was developed at 60°C for simultaneous quantification of allyl, 3-butenyl, 4-(methylthio)butyl, benzyl and phenethyl isothiocyanates. The validation parameter showed a very good linearity, with a correlation coefficient of 1.00 for all detected standard analytes. Also, high precision and accuracy were observed with lowest obtained values of 1.39% and 99.1%, respectively. Different varieties of three plants, viz. Brassica rapa var. rapa L., Raphanus sativus L. var. oleiformis Pers. and Eruca sativa Mill., were analyzed with this method. After analysis, 4-(methylthio)butyl isothiocyanate was observed to be the major component in the varieties of arugula. Allyl, benzyl and phenethyl isothiocyanates were detected in turnip varieties and, in addition, 3-butenyl isothiocyanate was detected in radish varieties.
在本研究中，反相超高效液相色谱 - 光电二极管阵列（UHPLC - PDA）技术于60°C下开发，用于同时定量烯丙基、3 - 丁烯基、4 - （甲硫基）丁基、苄基和苯乙基异硫氰酸酯。验证参数显示线性关系非常良好，所有检测到的标准分析物的相关系数均为1.00。此外，观察到高精度和准确性，最低获得值分别为1.39%和99.1%。使用该方法分析了三种植物的不同品种，即芜菁（Brassica rapa var. rapa L.）、油用萝卜（Raphanus sativus L. var. oleiformis Pers.）和芝麻菜（Eruca sativa Mill.）。分析后发现，4 - （甲硫基）丁基异硫氰酸酯是芝麻菜品种中的主要成分。在芜菁品种中检测到烯丙基、苄基和苯乙基异硫氰酸酯，此外，在萝卜品种中检测到3 - 丁烯基异硫氰酸酯。
