Kaur Harneetpal, Kaur Kirandeep, Singh Atamjit, Bedi Neena, Singh Balbir, Alturki Mansour S, Aldawsari Mohammed F, Almalki Atiah H, Haque Shafiul, Lee Hae-Jeung, Yadav Dharmendra K, Arora Saroj
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, India.
Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab, India.
Front Pharmacol. 2022 Oct 18;13:1020602. doi: 10.3389/fphar.2022.1020602. eCollection 2022.
Nanoformulation-based combinational drug delivery systems are well known to overcome drug resistance in cancer management. Among them, nanoemulsions are well-known and thermodynamically stable drug delivery systems suitable for carrying hydrophobic drugs and phytoconstituents to tackle drug-resistant cancers. In the present study, we have investigated the effect of paclitaxel in combination with erucin (natural isothiocyanate isolated from the seeds of ) loaded in the frankincense oil-based nanoemulsion formulation. The choice of frankincense oil for the current study was based on reported research investigations stating its magnificient therapeutic potential against breast cancer. Optimized nanoemulsion of paclitaxel (PTX) and erucin (ER) combination (EPNE) provided sustained release and exhibited enhanced cytotoxicity towards human epithelial breast cancer cells (T-47D) as compared to individual ER and PTX. EPNE was further assessed for its antitumor activity in the 7,12-dimethylbenz(a)anthracene (DMBA)-induced breast cancer mice model. EPNE significantly decreased the levels of hepatic and renal parameters along with oxidative stress in breast cancer mice. Furthermore, EPNE also showed decreased levels of inflammatory cytokines TNF-α, IL-6. Histopathological examinations revealed restoration of the tumorous breast to normal tissues in EPNE-treated breast cancer mice. Therefore, EPNE can act as a viable lead and therapeutic option for drug-resistant breast cancer.
基于纳米制剂的联合给药系统在癌症治疗中克服耐药性方面广为人知。其中，纳米乳剂是众所周知的且热力学稳定的给药系统，适用于携带疏水药物和植物成分来治疗耐药性癌症。在本研究中，我们研究了装载在乳香精油基纳米乳剂制剂中的紫杉醇与芥酸（从种子中分离出的天然异硫氰酸酯）联合使用的效果。本研究选择乳香精油是基于已报道的研究调查，该调查表明其对乳腺癌具有显著的治疗潜力。与单独的芥酸和紫杉醇相比，优化后的紫杉醇（PTX）和芥酸（ER）组合纳米乳剂（EPNE）提供了缓释效果，并对人上皮性乳腺癌细胞（T - 47D）表现出增强的细胞毒性。在7,12 - 二甲基苯并(a)蒽（DMBA）诱导的乳腺癌小鼠模型中进一步评估了EPNE的抗肿瘤活性。EPNE显著降低了乳腺癌小鼠的肝肾功能参数水平以及氧化应激。此外，EPNE还显示炎症细胞因子TNF-α、IL - 6的水平降低。组织病理学检查显示，EPNE治疗的乳腺癌小鼠的肿瘤性乳腺恢复为正常组织。因此，EPNE可以作为耐药性乳腺癌的一种可行的先导药物和治疗选择。
