Sangthong Sarita, Weerapreeyakul Natthida
Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand; Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Food Chem. 2016 Jun 15;201:139-44. doi: 10.1016/j.foodchem.2016.01.081. Epub 2016 Jan 20.
A simple, rapid and precise HPLC assay was developed for the well-known anti-cancer isothiocyanates-sulforaphene (SE) and sulforaphane (SF). The analytical system comprised RP-C18 column with isocratic 5% THF-95% water. High resolution was obtained (and eluted) of two distinct HPLC peaks of similar structures SE and SF analytes (at 23.01±0.02 and 25.65±0.03 min, respectively). The respective LOD vs. LOQ for SE and SF was 0.34 and 0.36 μg/ml vs. 1.02 and 1.08 μg/ml. This assay had the best linearity and accuracy. The recoveries were in the range of 96.83-101.17%. SF and SE were quantified in the pod of Raphanus sativus L. var. caudatus Alef extracts (2253.05±246.18 and 111.94±16.49 μg/g in the crude extract, respectively), while only SE was detected in the stem (1105.14±243.10 μg/g crude extract), as SF was lower than the detection limit. The validated method thus minimized and expedited simultaneous SE and SF analysis.
开发了一种简单、快速且精确的高效液相色谱（HPLC）分析方法，用于检测著名的抗癌异硫氰酸盐——萝卜硫素（SE）和萝卜硫烷（SF）。该分析系统由配备等度5%四氢呋喃 - 95%水的反相C18柱组成。获得了结构相似的SE和SF分析物的两个不同HPLC峰的高分辨率（并洗脱）（分别在23.01±0.02和25.65±0.03分钟）。SE和SF各自的检测限（LOD）与定量限（LOQ）分别为0.34和0.36μg/ml以及1.02和1.08μg/ml。该分析方法具有最佳的线性和准确性。回收率在96.83 - 101.17%范围内。在萝卜（Raphanus sativus L. var. caudatus Alef）提取物的荚中对SF和SE进行了定量（粗提取物中分别为2253.05±246.18和111.94±16.49μg/g），而在茎中仅检测到SE（粗提取物中为1105.14±243.10μg/g），因为SF低于检测限。因此，经过验证的方法最大限度地减少并加快了SE和SF的同时分析。
