Pohl Franziska, Goua Marie, Bermano Giovanna, Russell Wendy R, Scobbie Lorraine, Maciel Patrícia, Kong Thoo Lin Paul
School of Pharmacy and Life Science, Robert Gordon University, Aberdeen AB10 1GJ, Scotland, United Kingdom.
Centre for Obesity Research and Education (CORE), Robert Gordon University, Aberdeen AB10 1GJ, Scotland, United Kingdom.
Food Chem. 2018 Jan 15;239:323-332. doi: 10.1016/j.foodchem.2017.06.129. Epub 2017 Jun 23.
Rapeseed pomace (RSP) is a waste product obtained after edible oil production from Brassica napus. Analysis of ubiquitous secondary metabolites in RSP samples (two breeds, harvested in 2012/2014 respectively from North East of Scotland) and their ethanol/water (95:5) Soxhlet extracts were carried out. Soxhlet extraction of the RSP (petroleum ether followed by 95% ethanol) gave a solid extract. LC-MS/MS data of the extracts revealed several secondary metabolites, with Sinapic acid being the most abundant. Strong antioxidant activities of the Soxhlet extracts were confirmed from the results obtained in the FRAP, DPPH and ORAC assays. Furthermore, for the very first time, RSP extracts (13.9µg/ml) provided complete DNA protection, from oxidative stress induced by AAPH (3.5mM). Therefore the strong antioxidant and DNA protecting properties demonstrated by the RSP extracts in this study warrants further investigation for their revalorisation and potential use as reliable source of antioxidants in different food applications.
菜籽粕（RSP）是从油菜籽中提取食用油后产生的一种废弃物。对RSP样本（分别于2012年/2014年从苏格兰东北部收获的两个品种）及其乙醇/水（95:5）索氏提取物中普遍存在的次生代谢产物进行了分析。对RSP进行索氏提取（先用石油醚，再用95%乙醇）得到一种固体提取物。提取物的液相色谱-串联质谱数据显示了几种次生代谢产物，其中芥子酸含量最为丰富。从铁离子还原抗氧化能力（FRAP）、1,1-二苯基-2-三硝基苯肼（DPPH）和氧自由基吸收能力（ORAC）测定结果证实了索氏提取物具有较强的抗氧化活性。此外，RSP提取物（13.9µg/ml）首次提供了对由偶氮二异丁脒盐酸盐（AAPH，3.5mM）诱导的氧化应激的完全DNA保护。因此，本研究中RSP提取物所表现出的强大抗氧化和DNA保护特性值得进一步研究其再利用价值以及在不同食品应用中作为可靠抗氧化剂来源的潜在用途。
