You Xiangwei, Chen Xiaochu, Liu Fengmao, Hou Fan, Li Yiqiang
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, People's Republic of China; College of Science, China Agricultural University, Beijing 100193, People's Republic of China.
College of Science, China Agricultural University, Beijing 100193, People's Republic of China.
Food Chem. 2018 Jan 15;239:354-359. doi: 10.1016/j.foodchem.2017.06.074. Epub 2017 Jun 12.
A novel and simple ionic liquid-based air-assisted liquid-liquid microextraction technique combined with high performance liquid chromatography was developed to analyze five fungicides in juice samples. In this method, ionic liquid was used instead of a volatile organic solvent as the extraction solvent. The emulsion was formed by pulling in and pushing out the mixture of aqueous sample solution and extraction solvent repeatedly using a 10mL glass syringe. No organic dispersive solvent was required. Under the optimized conditions, the limits of detection (LODs) were 0.4-1.8μgL at a signal-to-noise ratio of 3. The limits of quantification (LOQs) set as the lowest spiking levels with acceptable recovery in juices were 10μgL, except for fludioxonil whose LOQ was 20μgL. The proposed method was applied to determine the target fungicides in juice samples, and acceptable recoveries ranging from 74.9% to 115.4% were achieved.
开发了一种新颖且简单的基于离子液体的空气辅助液液微萃取技术，并结合高效液相色谱法来分析果汁样品中的五种杀菌剂。在该方法中，使用离子液体代替挥发性有机溶剂作为萃取溶剂。通过使用10mL玻璃注射器反复吸入和推出水样溶液与萃取溶剂的混合物来形成乳液。无需有机分散溶剂。在优化条件下，信噪比为3时，检测限（LOD）为0.4 - 1.8μg/L。除咯菌腈的定量限（LOQ）为20μg/L外，在果汁中设定为具有可接受回收率的最低加标水平的定量限为10μg/L。所提出的方法应用于测定果汁样品中的目标杀菌剂，回收率在74.9%至115.4%之间，结果令人满意。
