Faculty of Fisheries Sciences, Hokkaido University, Minato 3, Hakodate, Hokkaido 041-8611, Japan.
Faculty of Fisheries Sciences, Hokkaido University, Minato 3, Hakodate, Hokkaido 041-8611, Japan.
Food Res Int. 2017 Oct;100(Pt 1):514-521. doi: 10.1016/j.foodres.2017.06.040. Epub 2017 Jul 14.
The use of dulse (Palmaria palmata) as a source of edible anti-inflammatory products was evaluated in this study. Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water-extraction, and subjected to thermolysin digestion to produce thermolysin-digested water-extract (d-DWE). d-DWE significantly reduced tumor necrosis factor-α, interleukin-6, and nitric oxide in LPS-stimulated murine macrophages (RAW 264.7 cells), and orally administered d-DWE mitigated acute inflammation in carrageenan-induced paw edema of mice. Mass spectrometry revealed d-DWE contained peptide LRDGEIILRY (derived from phycoerythrin β-chain) and chlorophyll a decomposition products, and they individually reduced the secretion of the proinflammatory mediators in LPS-stimulated RAW 264.7 cells. These results indicate the anti-inflammatory activity could be from a combined effect of phycobiliprotein and chlorophyll a decomposition products prepared from the water-extract of dulse. Thus, inexpensive and safe water-extraction method is effective for the extraction of anti-inflammatory components from dulse.
本研究评估了紫菜（Palmaria palmata）作为可食用抗炎产品的来源。通过水提的方法从冻干紫菜叶中同时提取藻蓝蛋白和叶绿素 a，然后用胰凝乳蛋白酶对其进行消化，得到胰凝乳蛋白酶水解的水提取物（d-DWE）。d-DWE 能显著降低脂多糖刺激的巨噬细胞（RAW 264.7 细胞）中肿瘤坏死因子-α、白细胞介素-6 和一氧化氮的水平，且口服 d-DWE 可减轻角叉菜胶诱导的小鼠足肿胀的急性炎症。质谱分析表明 d-DWE 含有肽 LRDGEIILRY（来源于藻红蛋白 β 链）和叶绿素 a 分解产物，它们分别能降低脂多糖刺激的 RAW 264.7 细胞中促炎介质的分泌。这些结果表明，抗炎活性可能来自于从紫菜的水提取物中制备的藻蓝蛋白和叶绿素 a 分解产物的协同作用。因此，从紫菜中提取抗炎成分的这种经济且安全的水提取方法是有效的。
