Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
Faculty of Sciences, Department of Chemistry, Selçuk University, Arciçh, Ismetpasa Cad, Selçuklu, Konya 42250, Turkey.
Mar Drugs. 2024 Oct 11;22(10):467. doi: 10.3390/md22100467.
A biorefinery process was developed to isolate phycobiliproteins, sulfated polysaccharides, and phenolic compounds from . The extraction process was carried out in three stages using ultrasound-assisted extraction (UAE) and pressurized liquid extraction (PLE) integrated with different natural deep eutectic solvents (NaDESs). In general, PLE provided higher phycobiliprotein contents than UAE in the first step of the process. In fact, the hydrolysis product of the PLE-NaDES extracts achieved a higher antioxidant capacity than that of the UAE-NaDES extracts. Particularly, glycerol:glucose (2:1) with 50% water in combination with PLE was the most suitable NaDES to recover the highest phycobiliprotein, protein, and sulfated polysaccharide contents from in the first and second steps of the biorefinery process. Finally, a PLE-NaDES using choline chloride:glycerol (1:2) with 60% water as the NaDES was employed for the recovery of antioxidant and neuroprotective phenolic compounds from the residue of the second step, obtaining a higher total phenolic content than employing PLE with ethanol/water (70:30, /) as the extraction solvent. Moreover, a forced stability study revealed that the NaDESs provided a protective effect compared to the water extracts against the degradation of phycobiliproteins, preserving their color over time. This study contributes to the recovery of high-value components from an undervalued biomarine source through a sustainable biorefinery process.
从开发了一种生物炼制工艺来分离藻蓝蛋白、硫酸多糖和酚类化合物。该提取过程分三个阶段进行，采用超声辅助提取（UAE）和加压液体提取（PLE）与不同的天然深共晶溶剂（NaDES）相结合。一般来说，在该过程的第一步中，PLE 比 UAE 提供了更高的藻蓝蛋白含量。事实上，PLE-NaDES 提取物的水解产物比 UAE-NaDES 提取物具有更高的抗氧化能力。特别是甘油：葡萄糖（2：1）与 50%的水相结合与 PLE 一起使用是从生物炼制过程的第一和第二步中回收最高藻蓝蛋白、蛋白质和硫酸多糖含量的最合适的 NaDES。最后，使用氯化胆碱：甘油（1：2）与 60%的水作为 NaDES 的 PLE 用于从第二步的残渣中回收抗氧化和神经保护酚类化合物，与使用乙醇/水（70：30，/）作为提取溶剂的 PLE 相比，获得了更高的总酚含量。此外，强制稳定性研究表明，与水提取物相比，NaDESs 提供了对藻蓝蛋白降解的保护作用，随着时间的推移保持其颜色。本研究通过可持续的生物炼制工艺从被低估的海洋生物源中回收高价值成分。
