垂体诱导怀孕的帚尾袋貂（Macropus eugenii）乳腺外植体泌乳：环磷酸腺苷的负面作用。

Pituitary-induced lactation in mammary gland explants from the pregnant tammar (Macropus eugenii): a negative role for cyclic AMP.

Maher F, Nicholas K R

Comp Biochem Physiol A Comp Physiol. 1987;87(4):1107-17. doi: 10.1016/0300-9629(87)90047-8.

Abstract

alpha-Lactalbumin and casein have been isolated from tammar milk. 2. alpha-Lactalbumin was induced in mammary explants by culture with anterior pituitary. 3. Casein was induced maximally in the presence of a physiological concentration of prolactin alone. 4. Progesterone did not inhibit the prolactin-induced synthesis of casein, alpha-lactalbumin, galactosyltransferase or fatty acids. 5. Both dibutyryl cAMP and a combination of cholera toxin and IBMX did significantly inhibit the induction of casein and alpha-lactalbumin. 6. Progesterone withdrawal is not a component of the lactogenic trigger in this marsupial but cAMP may be a common intracellular signal for negative control of lactogenesis in both marsupials and eutherians.

摘要

已从袋鼠奶中分离出α-乳白蛋白和酪蛋白。2. 通过与垂体前叶共同培养，乳腺外植体中可诱导产生α-乳白蛋白。3. 仅在生理浓度的催乳素存在时，酪蛋白的诱导量最大。4. 孕酮并不抑制催乳素诱导的酪蛋白、α-乳白蛋白、半乳糖基转移酶或脂肪酸的合成。5. 二丁酰环磷腺苷（dibutyryl cAMP）以及霍乱毒素与异丁基甲基黄嘌呤（IBMX）的组合均能显著抑制酪蛋白和α-乳白蛋白的诱导生成。6. 在这种有袋动物中，孕酮撤离并非泌乳触发机制的组成部分，但环磷腺苷（cAMP）可能是有袋动物和真兽类动物泌乳负调控的常见细胞内信号。