Perry J W, Oka T
In Vitro. 1984 Jan;20(1):59-65. doi: 10.1007/BF02633333.
The organ culture of the mammary gland of lactating mice was used to examine the response of the differentiated gland to lactogenic stimuli, insulin, cortisol, and prolactin. Time course studies showed that casein synthesis in cultured tissue decreased rapidly during the first 2 d despite the presence of the three hormones, but on the 3rd d tissue cultured with either insulin and prolactin or all three hormones regained the ability to synthesize milk proteins, casein, and alpha-lactalbumin: a greater increase occurred in the three hormone system. The delayed addition of prolactin on Day 2 to the culture system containing insulin and cortisol also stimulated casein synthesis. The addition of cytarabine, which inhibited insulin-dependent cell proliferation in cultured explants, did not block the rebound of milk protein synthesis. These results indicate that in the presence of insulin, cortisol, and prolactin mammary epithelial cells in culture first lose and then regain the ability of synthesizing milk protein without requiring the formation of new daughter cells.
利用泌乳小鼠乳腺的器官培养来检测分化的乳腺对生乳刺激物、胰岛素、皮质醇和催乳素的反应。时间进程研究表明，尽管存在这三种激素，但在培养的头两天，培养组织中的酪蛋白合成迅速下降，但在第三天，用胰岛素和催乳素或所有三种激素培养的组织恢复了合成乳蛋白、酪蛋白和α-乳白蛋白的能力：在三种激素系统中增加幅度更大。在第2天将催乳素延迟添加到含有胰岛素和皮质醇的培养系统中也刺激了酪蛋白合成。添加阿糖胞苷抑制了培养外植体中胰岛素依赖性细胞增殖，但并未阻止乳蛋白合成的反弹。这些结果表明，在存在胰岛素、皮质醇和催乳素的情况下，培养的乳腺上皮细胞首先丧失然后恢复合成乳蛋白的能力，而无需形成新的子细胞。
