National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai, 201306, China.
Sci Rep. 2017 Sep 5;7(1):10456. doi: 10.1038/s41598-017-10335-9.
High-throughput sequencing was applied to analyze the effects of toxic Microcystis aeruginosa on the silver carp Hypophthalmichthys molitrix. Silver carps were exposed to two cyanobacteria species (toxic and non-toxic) for RNA-seq and miRNA-seq analysis. RNA-seq revealed that the liver tissue contained 105,379 unigenes. Of these genes, 143 were significantly differentiated, 82 were markedly up-regulated, and 61 were remarkably down-regulated. GO term enrichment analysis indicated that 35 of the 154 enriched GO terms were significantly enriched. KEGG pathway enrichment analysis demonstrated that 17 of the 118 enriched KEGG pathways were significantly enriched. A considerable number of disease/immune-associated GO terms and significantly enriched KEGG pathways were also observed. The sequence length determined by miRNA-seq was mainly distributed in 20-23 bp and composed of 882,620 unique small RNAs, and 53% of these RNAs were annotated to miRNAs. As confirmed, 272 known miRNAs were differentially expressed, 453 novel miRNAs were predicted, 112 miRNAs were well matched with 7,623 target genes, and 203 novel miRNAs were matched with 15,453 target genes. qPCR also indicated that Steap4, Cyp7a1, CABZ01088134.1, and PPP1R3G were significantly differentially expressed and might play major roles in the toxic, detoxifying, and antitoxic mechanisms of microcystin in fish.
高通量测序被应用于分析毒性铜绿微囊藻对鲤鱼的影响。银鲤鱼暴露于两种蓝藻（有毒和无毒）进行 RNA-seq 和 miRNA-seq 分析。RNA-seq 显示肝组织包含 105379 个基因。其中，143 个基因差异显著，82 个基因显著上调，61 个基因显著下调。GO 术语富集分析表明，154 个富集的 GO 术语中有 35 个显著富集。KEGG 通路富集分析表明，118 个富集的 KEGG 通路中有 17 个显著富集。还观察到大量与疾病/免疫相关的 GO 术语和显著富集的 KEGG 通路。miRNA-seq 确定的序列长度主要分布在 20-23bp，由 882620 个独特的小 RNA 组成，其中 53%的 RNA 被注释为 miRNA。经证实，272 个已知 miRNA 表达差异，预测出 453 个新的 miRNA，112 个 miRNA 与 7623 个靶基因相匹配，203 个新的 miRNA 与 15453 个靶基因相匹配。qPCR 也表明 Steap4、Cyp7a1、CABZ01088134.1 和 PPP1R3G 表达差异显著，可能在鱼类微囊藻毒素的毒性、解毒和抗毒机制中发挥主要作用。
