Liu Wen-Jun, Cheng Ning-Bo, Meng Zhao-Qing, Fei Hong-Qiang, Sun Lin, Huang Wen-Zhe, Wang Zhen-Zhong, Wang Yue-Sheng, Xiao Wei
JiangSu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, China.
China Pharmaceutical University, Nanjing 210009, China.
Zhongguo Zhong Yao Za Zhi. 2016 Mar;41(5):887-890. doi: 10.4268/cjcmm20160521.
To discuss the synergistic mechanism of compatible use of two medicinal herbs,Panax notoginseng and Bletilla striata, an HPLC was established to determine two ginseng saponins (20S)-ginseng saponin Rg₃ and ginseng saponin Rh₄ contained in single decoction of Panax notoginseng as well as in compound decoction of Panax notoginseng and Bletillastriata in different compatibility ratio (1∶0.5, 1∶1, 1∶2), followed by analyzing the impact of amount of notoginsenosides after compatibility. As a result, compared with the single decoction of Panax notoginseng, the contents of ginseng saponin Rg₃ and ginseng saponin Rh₄ in the compound decoction of Panax notoginseng and Bletillastriata were on the rise as the increasement of the amount of Bletillastriata. The contents of the notoginsengsaponin R₁, ginseng saponin Rg₁ and ginseng saponin Rb₁ of Panax notoginseng single decoction were significantly decreased after compatibility. Therefore, after compatibility, it was more easy to produce (20S)-ginseng saponin Rg₃ and ginseng saponin Rh₄.This study can extend to a method of preparation of (20S)-ginseng saponin Rg₃ and ginseng saponin Rh₄. Furthermore, after compatibility, two ginseng saponins which had lipase inhibitory effect were both increased significantly, indicating that the compatibility of these two herb medicines may have effect on losing weight.
为探讨三七与白及配伍的协同作用机制，建立了高效液相色谱法测定三七单煎液及三七与白及按不同配伍比例（1∶0.5、1∶1、1∶2）的复方煎液中两种人参皂苷（20S）-人参皂苷Rg₃和人参皂苷Rh₄的含量，分析配伍后三七总皂苷含量的变化情况。结果表明，与三七单煎液相比，三七与白及复方煎液中人参皂苷Rg₃和人参皂苷Rh₄的含量随白及用量的增加而升高，三七单煎液中三七皂苷R₁、人参皂苷Rg₁和人参皂苷Rb₁的含量在配伍后显著降低。因此，配伍后更易生成（20S）-人参皂苷Rg₃和人参皂苷Rh₄。本研究可为（20S）-人参皂苷Rg₃和人参皂苷Rh₄的制备提供一种方法。此外，配伍后两种具有脂肪酶抑制作用的人参皂苷均显著增加，提示这两味中药配伍可能具有减肥作用。
