Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan.
J Periodontol. 2018 Jan;89(1):67-75. doi: 10.1902/jop.2017.170206.
Calprotectin, an inflammation-related protein, is present in gingival crevicular fluid (GCF), and the determination of calprotectin is useful for diagnosing periodontal diseases. The authors have recently developed a novel immunochromatographic (IC) chip system to determine calprotectin levels in GCF. In the present study, the usefulness of this diagnostic system is investigated in patients with periodontal diseases.
Thirty-six patients with periodontal diseases participated in this clinical test at multiple centers. Periodontitis sites (n = 118) and non-periodontitis (healthy) sites (n = 120) were selected after periodontal examination. GCF collection and periodontal examination were performed at baseline, after supragingival and subgingival scaling and root planing. Calprotectin levels in GCF were determined using a novel IC chip system and evaluated as a visual score and an IC reader value. Correlations between GCF calprotectin levels, clinical indicators, and changes in calprotectin levels by periodontal treatments were investigated. Receiver operating characteristic (ROC) analysis of IC reader value for GCF calprotectin was performed to predict periodontal diseases.
The visual score of GCF calprotectin was highly correlated with the IC reader value. IC reader values of GCF calprotectin in the periodontitis group were higher than those of the healthy group at three dental examination stages, and they significantly decreased with periodontal treatments. Visual scores and IC reader values of GCF calprotectin were correlated to levels of clinical indicators. ROC analysis for GCF calprotectin showed an optimal cutoff value to predict periodontal diseases.
Determination of GCF calprotectin using a novel IC chip system is useful for diagnosis of periodontal diseases.
钙卫蛋白是一种与炎症相关的蛋白，存在于龈沟液（GCF）中，其测定对于牙周病的诊断具有重要意义。作者最近开发了一种新的免疫层析（IC）芯片系统来测定 GCF 中的钙卫蛋白水平。本研究旨在探讨该诊断系统在牙周病患者中的应用价值。
本临床研究在多个中心招募了 36 名牙周病患者。在牙周检查后，选择牙周炎部位（n=118）和非牙周炎（健康）部位（n=120）。在基线、龈上洁治和龈下刮治及根面平整后，采集 GCF 并进行牙周检查。使用新型 IC 芯片系统测定 GCF 钙卫蛋白水平，并以目测评分和 IC 读数值进行评估。分析 GCF 钙卫蛋白水平与临床指标的相关性以及牙周治疗前后 GCF 钙卫蛋白水平的变化。对 IC 读数值进行 ROC 分析，以预测牙周病。
GCF 钙卫蛋白的目测评分与 IC 读数值高度相关。在三个牙检阶段，牙周炎组的 GCF 钙卫蛋白 IC 读数值均高于健康组，且经牙周治疗后显著降低。GCF 钙卫蛋白的目测评分和 IC 读数值与临床指标水平相关。ROC 分析显示，GCF 钙卫蛋白的最佳截断值可用于预测牙周病。
使用新型 IC 芯片系统测定 GCF 钙卫蛋白有助于牙周病的诊断。
