Kido J, Nakamura T, Kido R, Ohishi K, Yamauchi N, Kataoka M, Nagata T
Department of Periodontology and Endodontology, Tokushima University School of Dentistry, Kuramoto, Japan.
J Clin Periodontol. 1999 Oct;26(10):653-7. doi: 10.1034/j.1600-051x.1999.261004.x.
Clinical and biochemical markers of periodontal disease have been used for precise objective diagnosis of periodontal inflammation. Interleukin 1beta (IL-1beta) and prostaglandin E2 (PGE2), inflammatory factors, levels in gingival crevicular fluid (GCF) of patients with periodontal disease are elevated and have been studied as biochemical markers. The levels of calprotectin, a leukocyte protein, in body fluids of patients with some inflammatory diseases are raised. Recently, we detected calprotectin in GCF and its concentrations in periodontal pockets were higher than those in healthy gingival crevices. In this study, we investigated the correlations between GCF calprotectin levels and clinical indicators (probing depth and bleeding on probing, BOP), and the IL-1beta or PGE2 levels in GCE Probing depth and BOP at 130 sites of 110 subjects with periodontal or other oral diseases were examined, then GCF samples were collected and their calprotectin, IL-1beta and PGE2 were determined by ELISA. The calprotectin level correlated positively with the probing depth and was significantly higher at BOP-positive than BOP-negative sites. There were significant, positive correlations between the calprotectin and IL-1beta or PGE2 concentrations. These results indicate that the calprotectin level in GCF correlates well with clinical and biochemical markers of periodontal disease and suggest that calprotectin may be useful for evaluating the extent of periodontal inflammation.
牙周疾病的临床和生化标志物已被用于牙周炎症的精确客观诊断。白细胞介素1β（IL-1β）和前列腺素E2（PGE2）作为炎症因子，在牙周疾病患者的龈沟液（GCF）中的水平升高，并已作为生化标志物进行研究。在一些炎症性疾病患者的体液中，白细胞蛋白钙卫蛋白的水平会升高。最近，我们在龈沟液中检测到了钙卫蛋白，并且其在牙周袋中的浓度高于健康牙龈沟中的浓度。在本研究中，我们调查了龈沟液钙卫蛋白水平与临床指标（探诊深度和探诊出血，BOP）之间的相关性，以及龈沟液中IL-1β或PGE2的水平。对110名患有牙周疾病或其他口腔疾病的受试者的130个位点进行探诊深度和BOP检查，然后收集龈沟液样本，并通过酶联免疫吸附测定法测定其中的钙卫蛋白、IL-1β和PGE2。钙卫蛋白水平与探诊深度呈正相关，在探诊出血阳性位点显著高于探诊出血阴性位点。钙卫蛋白与IL-1β或PGE2浓度之间存在显著的正相关。这些结果表明，龈沟液中的钙卫蛋白水平与牙周疾病的临床和生化标志物密切相关，并提示钙卫蛋白可能有助于评估牙周炎症的程度。
