[鸡腿蘑多糖的分离、纯化及抗氧化活性]
[Separation, purification and antioxidant activity of polysaccharide from Coprinus comatus].
作者信息
Xu Nü, Zhang Tianzhen, Chen Xufeng, Zhang Hao, Wang Rufu
机构信息
Food Science and Engineering College, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2017 May 25;33(5):808-816. doi: 10.13345/j.cjb.160440.
We compared the ways of deproteinization for crude polysaccharides of Coprinus comatus, and finally selected Sevage method as the optimal method. Two main fractions of Ccp-I-A and Ccp-I-B were obtained after DEAE-52 cellulose and Sephadex G-200 chromatography, both were white-floc, soluble in water, insoluble in absolute ethyl alcohol, acetone and other organic solvents. Additionally, Fehling reagent, CTAB, Sulphuric acid-carbazole, I-KI and FeCl₃ reaction were all negative. GC analysis showed Ccp-I-A was composed of mannitose, glucose and galactose in molar ratios of 2.03:9.52:1, whereas Ccp-I-B was composed of fucose and galactose with molar ratios of 1:5.21. Antioxidant activity test showed that Ccp-I-A and Ccp-I-B had good scavenging abilities on DPPH and ·OH. Compared to Ccp-I-B,the scavenging activity of Ccp-I-A was much stronger, and the scavenging rate could reach 72.1% and 55.3% respectively when the concentration was 300 μg/mL.
我们比较了鸡腿蘑粗多糖的脱蛋白方法，最终选择 Sevage 法作为最佳方法。经 DEAE - 52 纤维素柱和 Sephadex G - 200 柱层析后，得到两个主要组分 Ccp - I - A 和 Ccp - I - B，均为白色絮状，可溶于水，不溶于无水乙醇、丙酮等有机溶剂。此外，与费林试剂、十六烷基三甲基溴化铵、硫酸 - 咔唑、碘化钾和三氯化铁的反应均为阴性。气相色谱分析表明，Ccp - I - A 由甘露糖、葡萄糖和半乳糖组成，摩尔比为 2.03:9.52:1，而 Ccp - I - B 由岩藻糖和半乳糖组成，摩尔比为 1:5.21。抗氧化活性测试表明，Ccp - I - A 和 Ccp - I - B 对 DPPH 和·OH 具有良好的清除能力。与 Ccp - I - B 相比，Ccp - I - A 的清除活性更强，当浓度为 300 μg/mL 时，清除率分别可达 72.1%和 55.3%。
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