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优化液体发酵条件以提高 的抗氧化活性。

Optimization of liquid fermentation conditions for to enhance antioxidant activity.

机构信息

School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian, China.

College of Marine Science and Environment, Dalian Ocean University, Dalian, China.

出版信息

Prep Biochem Biotechnol. 2024 Jul;54(6):830-837. doi: 10.1080/10826068.2023.2297703. Epub 2023 Dec 26.

Abstract

is an edible and medicinal fungus. In this study, the antioxidant activity of the fermentation product of was investigated through optimization of fermentation process. The results indicated that the fermentation product of had obvious scavenging ability for 2,2'-Azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) free radical. The EC of the -butanol extract from the fermentation product on ABTS· was 0.65 ± 0.02 mg/mL. On this basis, the liquid fermentation conditions of were optimized through single factor and response surface optimization experiments according to the scavenging ability of ABTS· to improve the antioxidant capacity of the fermentation product. The results showed that when the 14% of was fermented in a culture medium with a C/N ratio of 48:1 for 6 days, the ABTS· scavenging ability was the strongest, and the EC of -butanol extract was 0.57 ± 0.01 mg/mL, which was 12.31% higher than the initial activity. This study laid the foundation for the development of .

摘要

是一种食用兼药用真菌。本研究通过优化发酵工艺,对发酵产物的抗氧化活性进行了研究。结果表明,发酵产物对 2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基具有明显的清除能力。发酵产物的 -丁醇提取物对 ABTS·的 EC 为 0.65±0.02mg/mL。在此基础上,根据 ABTS·清除能力,采用单因素和响应面优化实验对 的液体发酵条件进行了优化,以提高发酵产物的抗氧化能力。结果表明,当 14%的 在 C/N 比为 48:1 的培养基中发酵 6 天时,ABTS·的清除能力最强,-丁醇提取物的 EC 为 0.57±0.01mg/mL,比初始活性提高了 12.31%。本研究为 的开发奠定了基础。

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