用于金黄色葡萄球菌中SprC对基因调控的相对和绝对定量蛋白质组学分析的等压标签
Isobaric tags for relative and absolute quantitation proteomics analysis of gene regulation by SprC in Staphylococcus aureus.
作者信息
Zhao Huanqiang, Hu Fupin, Yang Han, Ding Baixing, Xu Xiaogang, He Chunyan, Cui Zelin, Shu Wen, Liu Qingzhong
机构信息
Department of Clinical Laboratory, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.
出版信息
Future Microbiol. 2017 Oct;12:1181-1199. doi: 10.2217/fmb-2017-0033. Epub 2017 Sep 6.
AIM
To explore the complete gene networks regulated by small RNA SprC and its targets in Staphylococcus aureus.
MATERIALS & METHODS: The isobaric tags for relative and absolute quantitation and bioinformatic methods were utilized to identify and analyze the target proteins affected by SprC in S. aureus N315.
RESULTS
Proteomic analysis showed that the expression of 44 proteins was modulated by SprC. Further, bioinformatic analysis displayed that these affected proteins mainly associated with metabolic and cellular process, biological regulation and catalytic activity.
CONCLUSION
Our data provide a rich resource of SprC targets in S. aureus, although the mechanism of regulation by SprC is yet to be elucidated.
目的
探索小RNA SprC及其在金黄色葡萄球菌中的靶标所调控的完整基因网络。
材料与方法
采用相对和绝对定量的等压标签及生物信息学方法，鉴定和分析SprC对金黄色葡萄球菌N315中受影响的靶蛋白。
结果
蛋白质组学分析表明，44种蛋白质的表达受SprC调控。此外，生物信息学分析显示，这些受影响的蛋白质主要与代谢和细胞过程、生物调节及催化活性相关。
结论
我们的数据提供了丰富的金黄色葡萄球菌中SprC靶标的资源，尽管SprC的调控机制尚待阐明。
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