Wang Xiaocui, Zhu Peiyan, Qu Shanshan, Zhao Jie, Singh Prashant K, Wang Wei
Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng, Henan 475004, People's Republic of China.
Henan University Minsheng College, Kaifeng, Henan 475004, People's Republic of China.
Acta Crystallogr F Struct Biol Commun. 2017 Sep 1;73(Pt 9):532-535. doi: 10.1107/S2053230X1701250X. Epub 2017 Aug 31.
Plasmodesmata-localized protein 5 (PDLP5) is a cysteine-rich receptor-like protein which is localized on the plasmodesmata of Arabidopsis thaliana. Overexpression of PDLP5 can reduce the permeability of the plasmodesmata and further affect the cell-to-cell movement of viruses and macromolecules in plants. The ectodomain of PDLP5 contains two DUF26 domains; however, the function of these domains is still unknown. Here, the ectodomain of PDLP5 from Arabidopsis was cloned and overexpressed using an insect expression system and was then purified and crystallized. X-ray diffraction data were collected to 1.90 Å resolution and were indexed in space group P1, with unit-cell parameters a = 41.9, b = 48.1, c = 62.2 Å, α = 97.3, β = 103.1, γ = 99.7°. Analysis of the crystal content indicated that there are two molecules in the asymmetric unit, with a Matthews coefficient of 2.51 Å Da and a solvent content of 50.97%.
胞间连丝定位蛋白5（PDLP5）是一种富含半胱氨酸的类受体蛋白，定位于拟南芥的胞间连丝上。过表达PDLP5会降低胞间连丝的通透性，进而影响植物中病毒和大分子的细胞间移动。PDLP5的胞外结构域包含两个DUF26结构域；然而，这些结构域的功能仍然未知。在此，利用昆虫表达系统克隆并过表达了拟南芥PDLP5的胞外结构域，随后进行纯化和结晶。收集到分辨率为1.90 Å的X射线衍射数据，其属于空间群P1，晶胞参数为a = 41.9、b = 48.1、c = 62.2 Å，α = 97.3°、β = 103.1°、γ = 99.7°。晶体含量分析表明，不对称单元中有两个分子，马修斯系数为2.51 Å Da，溶剂含量为50.97%。
