School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, PR China.
ACS Appl Mater Interfaces. 2017 Sep 27;9(38):33011-33019. doi: 10.1021/acsami.7b10548. Epub 2017 Sep 14.
In this work, a new kind of N, Cu codoped carbon dots (N/Cu-CDs) was prepared via a facile one-pot hydrothermal method by using citric acid monohydrate, copper acetate monohydrate and diethylenetriamine. The prepared N/Cu-CDs with a high quantum yield (50.1%) showed excitation-independent emission at 460 nm. The structure and fluorescence properties of N/Cu-CDs were characterized by high-resolution transmission electron microscopy, fluorescence spectrofluorometer, FT-IR spectrometer, UV-visible spectrophotometer and X-ray photoelectron spectroscopy. N/Cu-CDs were applied to establishing a ratiometric fluorescence probe toward HO based on the inner filter effect (IFE) between N/Cu-CDs and DAP (2,3-diaminophenazine, the oxidative product of o-phenylenediamine (OPD)), and provided a ratiometric fluorescence universal platform for detection of the metabolites participating in HO-generation reactions (cholesterol and xanthine). The proposed method was demonstrated to be ultrasensitive and highly selective for cholesterol and xanthine assay with detection limits of 0.03 and 0.10 μM, respectively. The fluorescence probe built was applied to the determination of cholesterol and xanthine in human serum with satisfactory results.
在这项工作中，通过简便的一锅水热法，以一水柠檬酸、一水乙酸铜和二乙烯三胺为原料，制备了一种新型的 N、Cu 共掺杂碳点（N/Cu-CDs）。所制备的 N/Cu-CDs 具有高量子产率（50.1%），在 460nm 处表现出激发独立发射。通过高分辨率透射电子显微镜、荧光分光光度计、傅里叶变换红外光谱仪、紫外-可见分光光度计和 X 射线光电子能谱对 N/Cu-CDs 的结构和荧光性质进行了表征。N/Cu-CDs 被应用于建立基于 HO 的比率荧光探针，该探针基于 N/Cu-CDs 和 DAP（2,3-二氨基吩嗪，邻苯二胺（OPD）的氧化产物）之间的内滤效应（IFE），并为检测参与 HO 生成反应的代谢物（胆固醇和黄嘌呤）提供了比率荧光通用平台。所提出的方法对胆固醇和黄嘌呤的测定具有超灵敏和高度选择性，检测限分别为 0.03 和 0.10μM。该荧光探针被用于人血清中胆固醇和黄嘌呤的测定，结果令人满意。
