Huang Shan, Yang Erli, Yao Jiandong, Chu Xu, Liu Yi, Zhang Yue, Xiao Qi
Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, College of Chemistry and Materials, Nanning Normal University, 175 Mingxiu East Road, Nanning 530001, P. R. China.
Department of Food Science and Technology, University of Nebraska-Lincoln, 270 Food Innovation Center, Lincoln, Nebraska 68588, United States.
ACS Omega. 2019 May 28;4(5):9333-9342. doi: 10.1021/acsomega.9b00874. eCollection 2019 May 31.
Detection of cholesterol and uric acid biomarkers is of great importance for clinical diagnosis of several serious diseases correlated with their variations in human blood serum. In this study, a new kind of well selective and highly sensitive ratiometric fluorescent probe for cholesterol and uric acid determination in human blood serum was innovatively developed on the basis of the inner filter effect (IFE) process of nitrogen, cobalt co-doped carbon dots (N,Co-CDs) with 2,3-diaminophenazine (DAP). DAP was the oxidative product during the oxidation reaction between -phenylenediamine and HO. Fluorescent magnetic N,Co-CDs possessing blue emission and magnetic property were prepared through a facile one-pot hydrothermal strategy by using citric acid, diethylenetriamine, and cobalt(II) chloride hexahydrate as precursors. N,Co-CDs exhibited good ferromagnetic property and excellent optical properties even in extremely harsh environmental conditions, implying the huge potential applications of such N,Co-CDs in biological areas. On the basis of the IFE process between N,Co-CDs and DAP, N,Co-CDs were applied to establish ratiometric fluorescent probes for the indirect detection of cholesterol and uric acid that participated in enzyme-catalyzed HO-generation reactions. The established IFE-based fluorescent probes exhibited relatively low detection limits of 3.6 nM for cholesterol and 3.4 nM for uric acid, respectively. The fluorescent probe was successfully utilized for the determination of cholesterol and uric acid in human blood serum with satisfying results, which provided an informed perspective on the applications of such doped CDs to explore the specific and sensitive nanoprobe in disease diagnoses and clinical therapy.
检测胆固醇和尿酸生物标志物对于临床诊断与人类血清中其变化相关的几种严重疾病具有重要意义。在本研究中，基于氮、钴共掺杂碳点（N,Co-CDs）与2,3-二氨基吩嗪（DAP）的内滤效应（IFE）过程，创新性地开发了一种新型的用于检测人血清中胆固醇和尿酸的高选择性、高灵敏度比率荧光探针。DAP是对苯二胺与HO氧化反应过程中的氧化产物。以柠檬酸、二乙烯三胺和六水合氯化钴为前驱体，通过简便的一锅水热法制备了具有蓝色发射和磁性的荧光磁性N,Co-CDs。即使在极端恶劣的环境条件下，N,Co-CDs仍表现出良好的铁磁性能和优异的光学性能，这意味着此类N,Co-CDs在生物领域具有巨大的潜在应用价值。基于N,Co-CDs与DAP之间的IFE过程，将N,Co-CDs应用于构建比率荧光探针，用于间接检测参与酶催化HO生成反应的胆固醇和尿酸。所建立的基于IFE的荧光探针分别对胆固醇和尿酸表现出相对较低的检测限，分别为3.6 nM和3.4 nM。该荧光探针成功用于人血清中胆固醇和尿酸的测定，结果令人满意，为探索此类掺杂碳点在疾病诊断和临床治疗中特异性和灵敏性纳米探针的应用提供了有价值的视角。
