Wang Jixiang, Qin Yaowei, Ma Yue, Meng Minjia, Xu Yeqing
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200438, China.
Molecules. 2024 Dec 13;29(24):5888. doi: 10.3390/molecules29245888.
With the development and improvement of analysis and detection systems, low-toxicity and harmless detection systems have received much attention, especially in the field of food detection. In this paper, a low-toxicity dual-emission molecularly imprinted fluorescence sensor (CdTe QDs@SiO/N-CDs@MIPs) was successfully designed for highly selective recognition and visual detection of tetracycline (TC) in food samples. Specifically, the non-toxic blue-emission N-doped carbon dots (N-CDs) with high luminous performance acted as the response signals to contact TC via the covalent bond between amino and carboxyl groups. The red-emission CdTe quantum dots (CdTe QDs) were coated in silica nanospheres as stable reference signals, which effectively avoided the direct contact of CdTe QDs. Under optimum conditions, CdTe QDs@SiO/N-CDs@MIPs had a rapid response within 1.0 min to TC, and the detection limit of CdTe QDs@SiO/N-CDs@MIPs was calculated at 0.846 μM in the linear range of 0-140 μM. In complex environments, the CdTe QDs@SiO/N-CDs@MIPs also exhibited excellent capabilities for the selective, rapid, and visual detection of TC. Furthermore, the accuracy of CdTe QDs@SiO/N-CDs@MIPs to detect TC was verified by the HPLC method, and satisfactory results were obtained. Moreover, CdTe QDs@SiO/N-CDs@MIPs showed a satisfactory recovery when measuring TC in milk and egg samples. This work provided an ideal approach for low-toxicity fluorescence sensor design and application.
随着分析检测系统的发展与完善，低毒无害的检测系统备受关注，尤其是在食品检测领域。本文成功设计了一种低毒双发射分子印迹荧光传感器（CdTe量子点@SiO/N-碳点@分子印迹聚合物），用于食品样品中四环素（TC）的高选择性识别和可视化检测。具体而言，具有高发光性能的无毒蓝色发射氮掺杂碳点（N-碳点）作为响应信号，通过氨基和羧基之间的共价键与TC接触。红色发射的CdTe量子点（CdTe量子点）包覆在二氧化硅纳米球中作为稳定的参考信号，有效避免了CdTe量子点的直接接触。在最佳条件下，CdTe量子点@SiO/N-碳点@分子印迹聚合物对TC在1.0分钟内有快速响应，其检测限在0-140μM线性范围内计算为0.846μM。在复杂环境中，CdTe量子点@SiO/N-碳点@分子印迹聚合物对TC的选择性、快速和可视化检测也表现出优异的能力。此外，通过高效液相色谱法验证了CdTe量子点@SiO/N-碳点@分子印迹聚合物检测TC的准确性，并获得了满意的结果。此外，CdTe量子点@SiO/N-碳点@分子印迹聚合物在牛奶和鸡蛋样品中测定TC时显示出令人满意的回收率。这项工作为低毒荧光传感器的设计和应用提供了一种理想的方法。
