Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan.
Department of Chemistry and Biochemistry, National Institute of Technology, Suzuka College, Suzuka, Mie, Japan.
Small GTPases. 2020 Mar;11(2):146-154. doi: 10.1080/21541248.2017.1356425. Epub 2017 Nov 30.
Fission yeast Ypt2, an orthologue of the mammalian small GTPase Rab8, is responsible for post-Golgi membrane trafficking. During meiosis, Ypt2 localizes at the spindle pole body (SPB), where it regulates biogenesis of the spore plasma membrane. Recruitment of Ypt2 to the SPB is dependent on its meiosis-specific GDP/GTP exchange factor (GEF), the SPB-resident protein Spo13. Here we have examined the SPB recruitment of Ypt2 by Spo13. The GEF activity of Spo13 was required, but not essential for recruitment. Furthermore, Ypt2 recruitment was regulated in a meiosis-specific manner and partially regulated by the nuclear Dbf2-related (NDR) kinase Sid2, indicating the existence of a novel regulatory mechanism for localization of Rab GTPases during meiosis.
裂殖酵母 Ypt2 是哺乳动物小 GTP 酶 Rab8 的同源物，负责高尔基体后膜运输。在减数分裂过程中，Ypt2 定位于纺锤体极体 (SPB)，在那里它调节孢子质膜的生物发生。Ypt2 被招募到 SPB 取决于其减数分裂特异性 GDP/GTP 交换因子 (GEF)，即 SPB 驻留蛋白 Spo13。在这里，我们研究了 Spo13 对 Ypt2 的 SPB 招募。Spo13 的 GEF 活性是必需的，但不是招募所必需的。此外，Ypt2 的招募受到减数分裂特异性调节，部分受到核 Dbf2 相关 (NDR) 激酶 Sid2 的调节，表明在减数分裂过程中 Rab GTP 酶定位存在一种新的调节机制。
