VERIFIN, Finnish Institute for Verification of The Chemical Weapons Convention, Department of Chemistry, University of Helsinki , P.O. Box 55, FI-00014 Helsinki, Finland.
Anal Chem. 2017 Oct 17;89(20):11129-11134. doi: 10.1021/acs.analchem.7b03429. Epub 2017 Sep 25.
A new method has been developed to determine oxidation products of three chemical warfare agent (CWA) related phenylarsenic compounds from marine biota samples by a liquid chromatography-heated electrospray ionization/tandem mass spectrometry (LC-HESI/MS/MS). The target chemicals were oxidation products of Adamsite (DM[ox]), Clark I (DPA[ox]), and triphenylarsine (TPA[ox]). Method was validated within the concentration range of 1-5, 0.2-5, and 0.2-5 ng/g for DM[ox], DPA[ox], and TPA[ox], respectively. The method was linear, precise and accurate. Limits of quantification (LOQ) were 2.0, 1.3, and 2.1 ng/g for DM[ox], DPA[ox], and TPA[ox], respectively. A total of ten fish samples and one lobster sample collected from near Swedish coast, Måseskär dumpsite were analyzed. Trace concentrations below LOQ values were detected in three samples and the elemental composition of oxidized form of Clark I and/or II was confirmed by LC-HESI/HRMS. To our knowledge, this is the first study that provides the presence of CWA related chemicals in marine biota samples.
一种新的方法已经被开发出来，用于通过液相色谱-加热电喷雾电离/串联质谱（LC-HESI/MS/MS）来测定海洋生物样品中三种与化学战剂（CWA）相关的苯胂化合物的氧化产物。目标化学物质是亚当氏剂（DM[ox]）、克拉克 I（DPA[ox]）和三苯胂（TPA[ox]）的氧化产物。该方法在 DM[ox]、DPA[ox]和 TPA[ox]的浓度范围为 1-5、0.2-5 和 0.2-5ng/g 内进行了验证。该方法具有线性、精确和准确的特点。DM[ox]、DPA[ox]和 TPA[ox]的定量限（LOQ）分别为 2.0、1.3 和 2.1ng/g。从瑞典沿海的马塞斯基尔垃圾场附近采集了十份鱼类样本和一份龙虾样本进行了分析。在三个样本中检测到低于 LOQ 值的痕量浓度，并且通过 LC-HESI/HRMS 证实了克拉克 I 和/或 II 的氧化形式的元素组成。据我们所知，这是首次在海洋生物样本中发现与化学战剂相关的化学物质。
