Fang Wei, Yang Yin-Jun, Guo Bao-Lin, Cen Shan
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029,China.
Zhongguo Zhong Yao Za Zhi. 2016 Apr;41(7):1251-1256. doi: 10.4268/cjcmm20160716.
Twelve compounds were obtained by phytochemical investigation of 70% EtOH ( containing 0.5%NH3•H2O )extract of the roots of Bupleurum marginatum var. stenophyllum. Based on comparison of their spectral data, including HR-ESI-MS, ¹H-NMR, ¹³C-NMR data, with those of the literature, their structures were elucidated as saikosaponin b2 (1), saikosaponin a(2), saikosaponin b1(3), saikosaponin d (4), hydroxysaikosaponin a (5), saikosaponin b3 (6), saikosaponin c(7),saikosaponin i (8), saikosaponin f (9), chikusaikosides Ⅱ(10), saikosaponin s (11), and saikosaponin I(12). All compounds belong to olean-type triterpenoid saponin and compounds 1, 3, 5, 8-9,11, and 12 were isolated from this plant for the first time. At a concentration of 20 μmol•L⁻¹, compounds 2, 4, 6, 8, 11 and 12 showed strong inhibition activity against influenza virus WSN33 with the inhibition rate of 91.3%,88.6%,53.4%,61.3%,77.3% and 57.4%,respectively.
对窄叶柴胡根的70%乙醇（含0.5%氨水）提取物进行植物化学研究，得到了12种化合物。通过将它们的光谱数据，包括高分辨电喷雾电离质谱（HR-ESI-MS）、¹H-核磁共振（¹H-NMR）、¹³C-核磁共振（¹³C-NMR）数据与文献数据进行比较，确定它们的结构分别为柴胡皂苷b2（1）、柴胡皂苷a（2）、柴胡皂苷b1（3）、柴胡皂苷d（4）、羟基柴胡皂苷a（5）、柴胡皂苷b3（6）、柴胡皂苷c（7）、柴胡皂苷i（8）、柴胡皂苷f（9）、北柴胡皂苷Ⅱ（10）、柴胡皂苷s（11）和柴胡皂苷I（12）。所有化合物均属于齐墩果烷型三萜皂苷，化合物1、3、5、8 - 9、11和12首次从该植物中分离得到。在浓度为20 μmol•L⁻¹时，化合物2、4、6、8、11和12对流感病毒WSN33表现出较强的抑制活性，抑制率分别为91.3%、88.6%、53.4%、61.3%、77.3%和57.4%。
