State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, China.
Cell Death Dis. 2017 Sep 7;8(9):e3035. doi: 10.1038/cddis.2017.433.
Oral squamous cell carcinoma (OSCC) is a leading cause of cancer-related deaths worldwide, with 500 000 new cases each year. However, the mechanisms underlying OSCC development are relatively unknown. In this study, matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)-based proteomic strategy was used to profile the differentially expressed peptides/proteins between OSCC tissues and their adjacent noncancerous tissues. Sixty-seven unique peptide peaks and five distinct proteins were identified with changed expression levels. Among them, LRP6 expression was found to be upregulated in OSCC tissues, and correlated with a cluster of clinicopathologic parameters, including smoking, drinking, tumor differentiation status, lymph node metastasis and survival time. Notably, knockdown of LRP6 inhibited the proliferation ability of OSCC cells. Furthermore, we demonstrated that the expression of LRP6 in OSCC cells is positively correlated with its downstream oncogene, FGF8. The present study suggests that LRP6 could be a potential biomarker for OSCC patients, and might further assist in the therapeutic decisions in OSCC treatment.
口腔鳞状细胞癌（OSCC）是全球癌症相关死亡的主要原因，每年有 50 万新发病例。然而，OSCC 发展的机制尚不清楚。在本研究中，采用基质辅助激光解吸电离成像质谱（MALDI-IMS）-基于蛋白质组学策略来分析 OSCC 组织与其相邻非癌组织之间差异表达的肽/蛋白。鉴定出 67 个独特的肽峰和 5 种不同的蛋白质，其表达水平发生改变。其中，LRP6 在 OSCC 组织中表达上调，并与一系列临床病理参数相关，包括吸烟、饮酒、肿瘤分化状态、淋巴结转移和生存时间。值得注意的是，LRP6 的敲低抑制了 OSCC 细胞的增殖能力。此外，我们证明了 LRP6 在 OSCC 细胞中的表达与其下游癌基因 FGF8 呈正相关。本研究表明，LRP6 可能是 OSCC 患者的潜在生物标志物，并可能进一步有助于 OSCC 治疗中的治疗决策。
