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使用高分压一氧化碳气体（PCO = 400 hPa）和高分压二氧化碳（PCO = 100 hPa）对离体大鼠心脏进行72小时保存、复苏及移植

Seventy-Two-Hour Preservation, Resuscitation, and Transplantation of an Isolated Rat Heart with High Partial Pressure Carbon Monoxide Gas (PCO = 400 hPa) and High Partial Pressure Carbon Dioxide (PCO = 100 hPa).

作者信息

Hatayama Naoyuki, Yoshida Yu, Seki Kunihiro

机构信息

Resonance Club Co., Ltd., Tokyo, Japan.

出版信息

Cell Transplant. 2012 Mar;21(2-3):623. doi: 10.3727/096368912X638982.

DOI:10.3727/096368912X638982
PMID:28880587
Abstract

The cardiac cavity of an isolated rat heart was filled with a Krebs-Henseleit (KH) solution, and the heart was hung in a high-pressure chamber. After the high-pressure chamber had been filled with a mixed gas (PCO = 400 hPa, PCO = 100 hPa, PO = 900 hPa, PHe = 5600 hPa) and preserved for 72 h, we performed a cervical ectopic heart transplantation on a recipient rat and resuscitated the preserved heart. This is the first incidence in the world of a mammalian organ having been successfully preserved and resuscitated after 72 h via a desiccation method.

摘要

将离体大鼠心脏的心腔充满Krebs-Henseleit（KH）溶液，然后将心脏悬挂在高压腔中。在高压腔中充入混合气体（PCO = 400 hPa，PCO = 100 hPa，PO = 900 hPa，PHe = 5600 hPa）并保存72小时后，我们对受体大鼠进行了颈部异位心脏移植，并使保存的心脏复苏。这是世界上首次通过干燥法成功保存并复苏哺乳动物器官达72小时的案例。

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